RED2, a brain-specific member of the RNA-specific adenosine deaminase family
RED2, a brain-specific member of the RNA-specific adenosine deaminase family
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DOI：
10.1074/jbc.271.50.31795
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发表时间：
1996-12-13
影响因子：
4.8
通讯作者：
Seeburg, PH
中科院分区：
文献类型：
--
作者：
Melcher, T;Maas, S;Seeburg, PH
哺乳动物 RNA 特异性腺苷脱氨酶 DRADA/dsRAD（别名 ADAR）和 RED1（别名 ADARB1）与脑表达的谷氨酸受体亚基前 mRNA 和丁型肝炎病毒反基因组 RNA 的位点选择性编辑有关。这些酶在许多（如果不是全部）组织中表达，预测了一种尚未被重视的现象。 我们现在报道了 RED2（别名 ADARB2）的 cDNA 分子克隆，RED2 是啮齿动物中 RNA 特异性腺苷脱氨酶家族的第三个成员，RED2 与 RED1 序列密切相关，但似乎仅在大脑中表达，在大脑中表达广泛，在大脑中达到最高水平。 嗅球和丘脑，RED2 与 RED1 的进一步不同之处在于具有 54 个残基的氨基末端延伸，其中包括富含精氨酸的基序。与 DRADA 和 RED1 不同，重组表达的 RED2 不会使延伸的合成 dsRNA 或 GluR-B 前 mRNA 中的腺苷脱氨，然而，RED1 和 RED2 的嵌合体编辑了 GluR-B Q/R 和 R/G 效率中等的位点，我们的数据表明 RED2 可以编辑具有不同结构特征的大脑特定转录本。
The mammalian RNA specific adenosine deaminases DRADA/dsRAD (alias ADAR) and RED1 (alias ADARB1) have been implicated in the site selective editing of brain-expressed pre-mRNAs for glutamate receptor subunits and of antigenomic RNA of hepatitis delta virus, These enzymes are expressed in many if not all tissues, predicting an as yet unappreciated significance for adenosine deamination-mediated recoding of gene transcripts in the mammalian organism, We now report the molecular cloning of cDNA for RED2 (alias ADARB2), a third member of the RNA-specific adenosine deaminase family in the rodent, RED2 is closely sequence-related to RED1 but appears to be expressed only in the brain, where expression is widespread reaching highest levels in olfactory bulb and thalamus, RED2 further differs from RED1 in having a 54-residue amino-terminal extension which includes an arginine rich motif, Different from DRADA and RED1, recombinantly expressed RED2 did not deaminate adenosines in extended synthetic dsRNA or in GluR-B pre-mRNA, However, a chimera of RED1 and RED2 edited the GluR-B Q/R and R/G sites with moderate efficiency, Our data suggest that RED2 may edit brain specific transcripts with distinct structural features.