Changes in Membrane Permeability of Winter Wheat Cells following Freeze-Thaw Injury as Determined by Nuclear Magnetic Resonance.
Changes in Membrane Permeability of Winter Wheat Cells following Freeze-Thaw Injury as Determined by Nuclear Magnetic Resonance.
复制标题
核磁共振测定冻融损伤后冬小麦细胞膜渗透性的变化。
DOI：
10.1104/pp.61.6.878
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发表时间：
1978
期刊：
影响因子：
7.4
通讯作者：
L. Gusta
中科院分区：
文献类型：
--
作者：
P. Chen;L. Gusta
研究了驯化和未驯化哈尔科夫冬小麦的核磁共振弛豫时间。冠状细胞和驯化的细胞聚集体，以确定膜的通透性是否因冷冻而改变。核磁共振水分信号的衰减由两个指数分量组成：一个短的来自细胞外的水，一个长的来自细胞内的水。对未驯化和驯化1周的牙冠进行缓慢的冻融处理，可以减少长松弛时间，提示膜损伤。未驯化和驯化的树冠在液氮中直接杀死也得到了类似的结果。驯化的冻死树冠和细胞聚集体的质膜对Mn(2+)的通透性显著增加。冷冻对植物组织的伤害似乎是一种膜相关现象，但与低水分驯化组织(树冠)相比，高含水率的非驯化组织(细胞团)受到的伤害更大。
Nuclear magnetic resonance (NMR) relaxation times were studied in acclimated and nonacclimated Kharkov winter wheat (Triticum aestivum L.) crowns and acclimated cell aggregates to determine if membrane permeability was altered by freezing. The NMR water signal decay consisted of two exponential components: a short one arising from extracellular water, and a long one arising from intracellular water. A slow freezethaw treatment of nonacclimated and 1-week acclimated crowns decreased the long relaxation time, suggesting membrane injury. Similar results were obtained for nonacclimated and acclimated crowns killed directly in liquid N(2).A significant increase in plasma membrane permeability to Mn(2+) was observed in acclimated freeze-killed crowns and cell aggregates. Freezing injury to plant tissue appears to be a membrane-related phenomenon, but more extensive injury occurs to nonacclimated and acclimated tissue with a high water content (cell aggregates) compared to acclimated tissue with a low water content (crowns).