Localization and sex steroid regulation of androgen receptor gene expression in rhesus monkey uterus
Localization and sex steroid regulation of androgen receptor gene expression in rhesus monkey uterus
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DOI：
10.1016/s0029-7844(98)00384-6
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发表时间：
1999-02-01
影响因子：
7.2
通讯作者：
Bondy, C
中科院分区：
文献类型：
--
作者：
Adesanya-Famuyiwa, OO;Zhou, JA;Bondy, C
目的：探讨猴子宫雄激素受体基因表达的细胞位点和激素调控。方法：将切除卵巢的恒河猴（每组5只）分别给予安慰剂（对照组）、雌二醇（E2）、E2 +黄体酮或E2 +睾酮缓释微丸皮下注射。治疗3 d后，切除子宫，用原位杂交法检测子宫雄激素受体信使RNA （mRNA）。结果：雄激素受体mRNA在子宫内膜间质细胞和子宫内膜平滑肌细胞中均有表达，在子宫内膜上皮细胞中表达较少。E2和E2 +黄体酮均可使基质细胞雄激素受体mRNA水平增加一倍(P
Objective: To characterize the cellular sites and hormonal regulation of uterine androgen receptor gene expression in the monkey.Methods: Ovariectomized rhesus monkeys (five in each group) were treated with placebo (the control group), estradiol (E2), E2 plus progesterone, or E2 plus testosterone by sustained-release pellets administered subcutaneously. After 3 days of treatment, uteri were removed and uterine sections were analyzed by in situ hybridization for androgen receptor messenger RNA (mRNA).Results: Androgen receptor mRNA was detected in endometrial stromal cells and myometrial smooth muscle cells, with lesser expression in endometrial epithelial cells. Both E2 and E2 plus progesterone treatment doubled androgen receptor mRNA levels in stromal cells (P