Assignment of the carbon-13 nuclear magnetic resonance spectrum of folic acid.
Assignment of the carbon-13 nuclear magnetic resonance spectrum of folic acid.
复制标题
叶酸碳 13 核磁共振谱的分配。
DOI：
10.1021/bi00737a009
复制
发表时间：
1973
期刊：
影响因子：
2.9
通讯作者：
R. Dunlap
中科院分区：
文献类型：
--
作者：
J. Lyon;P. D. Ellis;R. Dunlap
给出并分析了D2Q中Pd为7.9的叶酸的质子去偶合和耦合~(13)C核磁共振谱。叶酸被认为是由三个组分组成的：L-谷氨酸、氨基苯甲酰胺和蝶啶环系，以帮助确定其~(13)C波谱。通过考察L-谷氨酸的~(13)C核磁共振谱和根据苯胺和苯甲酰胺的加成反应预测对氨基苯甲酰胺的化学位移，完成了对-氨基-苯甲酰-L-谷氨酸的~(13)C核磁共振谱的归属。通过与某些嘌呤核苷、喹唑啉、喹恶啉和4-羟基蝶啶的~(13)C核磁共振数据的类比，分析了叶酸的翼状结构部分。
Both the proton-decoupled and coupled 13C nmr spectra of folic acid in D2Q at pD 7.9 are presented and ana-lyzed. To aid in the assignment of its 13C resonances, folic acid was considered to be composed of three components: l-glutamic acid,/>-aminobenzamide, and the pteridine ring system. Assignment of the 13C nmr spectrum of Vp-amino-benzoyl-L-glutamate was accomplished by examination of both the 13C nmr spectrum of L-glutamic acid and the chemical shifts predicted for p-aminobenzamide on the basis of additivity for aniline and benzamide. The pteridinering portion of folic acid was analyzed by analogy with I3C nmr data for certain purine nucleosides, quinazoline, quinoxaline, and 4-hydroxypteridine.