Comparative proteomic profiling in Chinese shrimp Fenneropenaeus chinensis under low pH stress
Comparative proteomic profiling in Chinese shrimp Fenneropenaeus chinensis under low pH stress
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低pH胁迫下中国明对虾的蛋白质组比较分析
DOI：
10.1016/j.fsi.2021.12.032
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发表时间：
2021-12-23
影响因子：
4.7
通讯作者：
Li, Zhaoxia
中科院分区：
文献类型：
--
作者：
He, Yuying;Wang, Qiong;Li, Zhaoxia
较低的pH值对甲壳类动物产生有害的压力。本研究采用基于绝对定量的定量蛋白质组学（iTRAQ）方法，分析了对照pH值（8.2）和低pH值（6.5）处理组中国对虾的蛋白质组学响应。在鉴定的蛋白中，共有76个蛋白的丰度水平不同，其中45个蛋白表达上调，31个蛋白表达下调。柠檬酸合成酶、细胞色素c氧化酶、v型质子atp酶、甘油醛-3-磷酸脱氢酶和果糖1,6-二磷酸醛缩酶等蛋白的上调以及DEPs在多种代谢过程和途径中的富集表明，能量和底物代谢的增加是羊草抵抗低pH胁迫所必需的。Na+/K+/2Cl（-）共转运蛋白、钙调蛋白等离子转运相关蛋白参与了中国赤杨体内pH的稳态维持。凝集素、脂多糖和β -1,3-葡聚糖结合蛋白、几丁质酶、组织蛋白酶L和β -葡糖苷酸酶的表达均显著下调，表明低pH条件下中国赤霉素免疫功能紊乱。这些发现有助于我们进一步认识低pH胁迫的防御机制，并加快中国赤霉素耐低pH育种进程。
Lower pH gives rise to a harmful stress to crustacean. Here, we analyzed the proteomic response of Fenneropenaeus chinensis from control pH (pH value 8.2) and low pH (pH value 6.5) - treated groups by employing absolute quantitation-based quantitative proteomic (iTRAQ) analysis. Among the identified proteins, a total of 76 proteins differed in their abundance levels, including 45 upregulated and 31 downregulated proteins. The upregulation of proteins like citrate synthase, cytochrome c oxidase, V-type proton ATPase, glyceraldehyde-3-phosphate dehydrogenase and fructose 1,6-bisphosphate-aldolase as well as the enrichment of the DEPs in multiple metabolic processes and pathways illustrated that increased energy and substrates metabolism was essential for F. chinensis to counteract low pH stress. Ion transporting related proteins, such as Na+/K+/2Cl(-) cotransporter and calmodulin, participated in the homeostatic maintenance of pH in F. chinensis. There were significant downregulation expressions of lectin, lipopolysaccharide- and beta-1,3-glucan binding protein, chitinase, cathepsin L and beta-glucuronidase, which indicating the immune dysfunction of F. chinensis when exposure to low pH condition. These findings can extend our understanding on the defensive mechanisms of the low pH stress and accelerate the breeding process of low pH tolerance in F. chinensis.