HIGH-PERFORMANCE HYDROPHOBIC-INTERACTION CHROMATOGRAPHY OF PROTEINS ON A SERIES OF POLY(ALKYL ASPARTAMIDE)-SILICAS
HIGH-PERFORMANCE HYDROPHOBIC-INTERACTION CHROMATOGRAPHY OF PROTEINS ON A SERIES OF POLY(ALKYL ASPARTAMIDE)-SILICAS
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DOI：
10.1016/0021-9673(86)80064-4
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发表时间：
1986-05-30
期刊：
影响因子：
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通讯作者：
ALPERT, AJ
中科院分区：
文献类型：
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作者：
ALPERT, AJ
在二氧化硅表面制备了一系列键合型聚（烷基马来酰胺）涂层。该产品表现出广泛的疏水性，并进行了评价的高效疏水相互作用色谱（HPHIC）的蛋白质。聚（丙基乙酰胺）-二氧化硅是蛋白质HPHIC的良好通用材料;聚（乙基乙酰胺）-二氧化硅可用于更疏水的蛋白质。蛋白质的保留通常随着涂层中配体长度的增加而显著增加，但对于某些没有明确三级结构的载脂蛋白，情况并非如此。还观察到峰宽和构象不稳定性之间的相关性;载脂蛋白（极端情况）在异常宽的峰中洗脱。这些原理可用于获得新蛋白质物理特性的数据。这在rec A蛋白中得到了证实。
A series of bonded poly(alkyl aspartamide) coatings was prepared on silica. The products showed a wide range of hydrophobicity, and were evaluated for high-performance hydrophobic-interaction chromatography (HPHIC) of proteins. Poly-(propyl aspartamide)-silica is a good general-purpose material for protein HPHIC; poly(ethyl aspartamide)-silica may be useful for more hydrophobic proteins.Retention of proteins generally increased dramatically with ligand length in the coating, but this was not true of certain apolipoproteins with no well-defined tertiary structure. A correlation was also observed between peak width and conformational lability; the apolipoproteins (an extreme case) were eluted in exceptionally broad peaks. These principles can be used to obtain data on the physical characteristics of new proteins. This was demonstrated withrec Aprotein.