Transient developmental expression of monoamine transporters in the rodent forebrain.
Transient developmental expression of monoamine transporters in the rodent forebrain.
复制标题
啮齿动物前脑中单胺转运蛋白的瞬时发育表达。
DOI：
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发表时间：
1998
期刊：
影响因子：
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通讯作者：
P. Gaspar
中科院分区：
文献类型：
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作者：
C. Lebrand;O. Cases;R. Wehrlé;R. Blakely;R. Edwards;P. Gaspar
研究表明，丘脑一级感觉核中的神经元在出生后早期发育过程中表达功能性质膜5-羟色胺（SERT）和囊泡单胺（VMAT 2）转运蛋白。在本研究中，我们提供了一个广泛的描述的空间和时间模式的VMAT 2和SERT的表达，在早期胚胎发育和出生后的生活，通过使用原位杂交和免疫细胞化学。VMAT 2和SERT基因在中枢和外周神经系统中的广泛的非单胺能神经元群体中瞬时表达，两种基因的表达的时间和空间模式有很大的重叠。在丘脑中观察到这两种基因的选择性表达模式，其表达仅限于背侧丘脑，更特别地是传递点到点投影图的初级感觉中继核。在感觉脑神经、海马、大脑皮层、隔和杏仁核中也观察到转运蛋白的瞬时表达。VMAT 2和SERT基因表达不一定相关，因为一些神经群体仅表达VMAT 2，而另一些仅含有SERT。由于VMAT 2除了5-羟色胺外还负责转运儿茶酚胺，我们研究了质膜多巴胺和去甲肾上腺素转运蛋白的发育表达，但没有发现这些基因的瞬时表达。尽管存在微小的时间差异，但VMAT 2和SERT在出生后的第二周和第三周几乎同时熄灭。这些表达似乎并不依赖于外周神经的输入，因为单眼眼球摘除和眶下神经切割的影响，出生当天，没有修改期间的转运蛋白表达或灭绝。
Neurons in first-order sensory thalamic nuclei have been shown to express functional plasma membrane serotonin (SERT) and vesicular monoamine (VMAT2) transporters during early postnatal development. In the present study, we provide an extensive description of the spatial and the temporal patterns of VMAT2 and SERT expression, during early embryonic development and postnatal life, by using in situ hybridization and immunocytochemistry. VMAT2 and SERT genes are transiently expressed in a wide population of non-monoaminergic neurons in the central and peripheral nervous system with a large overlap in the temporal and spatial pattern of expression of both genes. A selective pattern of expression of both genes was observed in the thalamus with expression limited to the dorsal thalamus and more particularly to primary sensory relay nuclei that convey point to point projection maps. Transient expression of the transporters was also observed in sensory cranial nerves, in the hippocampus, cerebral cortex, septum, and amygdala. VMAT2 and SERT gene expression was not necessarily linked, as some neural populations expressed only VMAT2, while others only contained SERT. Since VMAT2 serves to transport catecholamines besides serotonin, we examined the developmental expression of the plasma membrane dopamine and norepinephrine transporters but found no transient expression of these genes. Despite minor temporal disparities, VMAT2 and SERT extinguished almost simultaneously during the second and third weeks of post-natal life. These expressions did not seem to be dependent on peripheral neural inputs, since monocular enucleations and infraorbital nerve cuts effected on the day of birth, did not modify the period of transporter expression or of extinction.