Re-innervation patterns by peptidergic Substance-P, non-peptidergic P2X3, and myelinated NF-200 nerve fibers in epidermis and dermis of rats with neuropathic pain
Re-innervation patterns by peptidergic Substance-P, non-peptidergic P2X3, and myelinated NF-200 nerve fibers in epidermis and dermis of rats with neuropathic pain
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DOI：
10.1016/j.expneurol.2012.11.029
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发表时间：
2013-03-01
影响因子：
5.3
通讯作者：
Walbeehm, Erik T.
中科院分区：
文献类型：
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作者：
Duraku, Liron S.;Hossaini, Mehdi;Walbeehm, Erik T.
表皮中的神经末梢，称为伤害感受器，将伤害性刺激的信息传导至中枢神经系统。然而，表皮神经纤维在神经病理性疼痛中的确切作用仍有争议。在这里，我们研究了在大鼠神经病理性疼痛模型中表皮和真皮神经纤维的再神经支配模式。应用备用神经损伤（SNI）模型后，我们测定了受影响后爪未受损伤的外侧（腓肠）和内侧（隐）区的机械和热退缩阈值，并研究了表皮和真皮中P物质（SubP）、神经丝-200（NF-200）和P2 X3免疫反应（IR）神经纤维的支配模式。术后2周，我们发现足底中央区域的肽能（SubP和NF-200）和非肽能（P2 X3）神经纤维的密度显著降低。与术后2周相比，术后10周表皮和真皮上层的Sub P-IR纤维密度以及真皮上层的P2 X3-IR纤维密度显著增加，但仍显著低于对照组的密度。然而，NF-200-IR纤维在中心区域的密度在PO 10周时达到对照水平。足底内侧部和外侧部的任何纤维的密度均未发现变化。本研究结果提示，周围神经损伤后，特定的神经纤维在表皮和真皮中具有不同的神经再支配模式，它们可能参与神经病理性疼痛的发展。(c)2012 Elsevier Inc. All rights reserved.
Nerve endings in the epidermis, termed nociceptors, conduct information on noxious stimuli to the central nervous system. The precise role of epidermal nerve fibers in neuropathic pain is however still controversial. Here, we have investigated the re-innervation patterns of epidermal and dermal nerve fibers in a rat neuropathic pain model. After applying the spared nerve injury (SNI) model, we determined the mechanical and thermal withdrawal thresholds in the uninjured lateral (sural) and medial (saphenous) areas of the affected hind paw and investigated the innervations patterns of Substance P (SubP), Neurofilament-200 (NF-200) and P2X3-immunoreactive (IR) nerve fibers in the epidermis and dermis. We found a significant loss in the density of peptidergic (SubP and NF-200) and non-peptidergic (P2X3) nerve fibers in the center area of the foot sole at 2 weeks postoperatively (PO). The densities of Sub P-IR fibers in the epidermis and upper dermis, and the density of P2X3-IR fibers in the upper dermis were significantly increased at 10 weeks PO as compared to 2 weeks PO, but were still significantly lower than the densities in controls. However, the density of NF-200-IR fibers in the center area reached control levels at 10 weeks PO. No changes were found in the densities of any of the fibers in the medial and lateral parts of the foot sole. The present results suggest that after peripheral nerve injury, specific nerve fibers have different re-innervation patterns in the epidermis and dermis and that they might be involved in the development of neuropathic pain. (c) 2012 Elsevier Inc. All rights reserved.