Programming Dynamic Assembly of Viral Proteins with DNA Origami
Programming Dynamic Assembly of Viral Proteins with DNA Origami
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用 DNA Origami 编程病毒蛋白的动态组装
DOI：
10.1021/jacs.9b13773
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发表时间：
2020
影响因子：
15
通讯作者：
Yonggang Ke
中科院分区：
文献类型：
--
作者：
Kun Zhou;Yihao Zhou;Victor Pan;Qiangbin Wang;Yonggang Ke
生物系统中的生物分子组装通常是由生物分子（如蛋白质和核酸）之间的分子信息交换调节的复杂动态过程。在这里，我们展示了一个基于核酸的系统，可以编程的病毒蛋白质的动态组装过程。烟草花叶病毒（TMV）基因组模拟RNA通过与一系列DNA链杂交而锚定在DNA折纸纳米结构上，所述DNA链也起到防止RNA被TMV蛋白包装的锁的作用。通过支点介导的链置换的RNA的选择性，顺序释放允许我们程序的RNA的可用性，随后的TMV原位生长。此外，TMV在DNA模板上的可编程动态组装还能够产生新的DNA-蛋白质混合纳米结构，这是通过使用先前的组装方法无法实现的。
Biomolecular assembly in biological systems is typically a complex dynamic process regulated by the exchange of molecular information between biomolecules such as proteins and nucleic acids. Here, we demonstrate a nucleic-acid-based system that can program the dynamic assembly process of viral proteins. Tobacco mosaic virus (TMV) genome-mimicking RNA is anchored on DNA origami nanostructures via hybridization with a series of DNA strands which also function as locks that prevent the packaging of RNA by the TMV proteins. The selective, sequential releasing of the RNA via toehold-mediated strand displacement allows us to program the availability of RNA and subsequently the TMV growth in situ. Furthermore, the programmable dynamic assembly of TMV on DNA templates also enables the production of new DNA–protein hybrid nanostructures, which are not attainable by using previous assembly methods.