Stable chemical bonding of porous membranes and poly(dimethylsiloxane) devices for long-term cell culture.
Stable chemical bonding of porous membranes and poly(dimethylsiloxane) devices for long-term cell culture.
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DOI：
10.1063/1.4883075
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发表时间：
2014-06
期刊：
影响因子：
3.2
通讯作者：
C. Sip;A. Folch
中科院分区：
文献类型：
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作者：
C. Sip;A. Folch
我们已经调查了各种硅烷处理集成的轨道蚀刻膜与聚（二甲基硅氧烷）（PDMS）的微流体器件的键合稳定性。我们比较各种治疗使用三烷氧基硅烷或dipodal硅烷，以确定的有机官能团，交联密度，反应溶剂，和催化剂上的键稳定性的影响。我们发现，使用现有硅烷方法制造的器械在浸入37 °C的细胞培养基中一天后分层。相比之下，双臂硅烷，双[3-（三甲氧基甲硅烷基）丙基]胺，显示出产生稳定的和功能性的膜与PDMS的整合，其适合于长期细胞培养。为了证明该技术的应用，我们制造了一个开放的表面装置，其中在径迹蚀刻膜上培养的细胞可以通过嵌入的微流体通道在其基底侧受到刺激。C2 C12小鼠成肌细胞在这些装置上在两周的过程中分化成肌管，以证明生物相容性。最后，在基底侧递送荧光染色剂期间对设备进行成像，以验证粘合技术的膜操作和长期稳定性。
We have investigated the bonding stability of various silane treatments for the integration of track-etched membranes with poly(dimethylsiloxane) (PDMS) microfluidic devices. We compare various treatments using trialkoxysilanes or dipodal silanes to determine the effect of the organofunctional group, cross-link density, reaction solvent, and catalyst on the bond stability. We find that devices made using existing silane methods delaminated after one day when immersed in cell culture medium at 37 °C. In contrast, the dipodal silane, bis[3-(trimethoxysilyl)propyl]amine, is shown to yield stable and functional integration of membranes with PDMS that is suitable for long-term cell culture. To demonstrate application of the technique, we fabricated an open-surface device in which cells cultured on a track-etched membrane can be stimulated at their basal side via embedded microfluidic channels. C2C12 mouse myoblasts were differentiated into myotubes over the course of two weeks on these devices to demonstrate biocompatibility. Finally, devices were imaged during the basal-side delivery of a fluorescent stain to validate the membrane operation and long-term stability of the bonding technique.