Influence of pericarp, cotyledon and inhibitory substances on sharp tooth oak (Quercus aliena var. acuteserrata) germination.
Influence of pericarp, cotyledon and inhibitory substances on sharp tooth oak (Quercus aliena var. acuteserrata) germination.
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DOI：
10.1371/journal.pone.0047682
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Li G
中科院分区：
文献类型：
--
作者：
Liu Y;Liu G;Li Q;Liu Y;Hou L;Li G
为探讨锐齿栎种子延迟萌发和不均匀萌发的机理。以锐齿藓（STO）为试材，采用机械划痕法研究了STO的根、芽萌发和生长情况。采用的技术有：去杯疤（RS）、去果皮（RP）、切去1/2（HC）和2/3（TC）子叶。以大白菜种子为试验材料，在三洋生长箱中分别用蒸馏水和80%的橡子胚、子叶和果皮甲醇提取液（橡子干重分别为1.0g、0.8g、0.6g和0.4g/ml甲醇）浇灌大白菜种子，研究了大白菜种子的发芽率和根冠长。结果表明，RP和HC处理的橡子大部分根和芽的出苗时间提前2周，出苗时间更同步，出苗总量分别提高46%和28%以上。TC处理促进了根、芽的出苗时间和根长，但根、芽的发芽率和苗高与对照差异不显著。RS治疗的所有指标均未观察到积极结果。用1.0 g·ml−1的胚和子叶甲醇提取物浇灌的CCS的发芽率显著低于果皮的发芽率，并且所有浓度均导致根和芽的生长下降。果皮甲醇提取物显著降低了CCS的根长，但对发芽率和芽长的影响不大。随着甲醇提取物浓度的增加，抑制作用逐渐增强。我们的结论是，果皮的机械限制和存在的发芽抑制剂在胚胎，子叶和果皮是延迟和异步萌发的STO橡子的原因。
In order to explore the mechanism of delayed and uneven germination in sharp tooth oak (Quercus aliena var. acuteserrata) (STO), mechanical scarification techniques were used to study STO root and shoot germination and growth. The techniques used were: removing cup scar (RS), removing the pericarp (RP), and cutting off 1/2 (HC) and 2/3 (TC) cotyledons. Germination percentage and root and shoot length for Chinese cabbage (Beassica pekinensis) seeds (CCS) were also investigated for CCS cultivated in a Sanyo growth cabinet watered by distilled water and 80% methanol extracts from the acorn embryo, cotyledon and pericarp with concentrations of 1.0 g, 0.8 g, 0.6 g and 0.4 g dry acorn weight per ml methanol. The results showed that the majority of roots and shoots from acorns with RP and HC treatment emerged two weeks earlier, more simultaneously, and their total emergences were more than 46% and 28% higher, respectively. TC accelerated root and shoot emergence time and root length, but root and shoot germination rate and shoot height had no significant difference from the control. Positive consequences were not observed on all indices of RS treatment. The germination rates of CCS watered by 1.0 g·ml−1 methanol extracts from the embryo and cotyledon were significantly lower than those from the pericarp, and all concentrations resulted in decreased growth of root and shoot. Methanol extracts from pericarp significantly reduced root length of CCS, but presented little response in germination percentage and shoot length. The inhibitory effect was gradually increased with the increasing concentration of the methanol extract. We conclude that both the mechanical restriction of the pericarp and the presence of germination inhibitors in the embryo, cotyledon and pericarp are the causes for delayed and asynchronous germination of STO acorns.
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影响因子：
2.1
作者：
Finch-Savage, W. E.;Clay, H. A.
通讯作者：
Clay, H. A.
影响因子：
2.2
作者：
Lombardo, Jeffrey A.;McCarthy, Brian C.
通讯作者：
McCarthy, Brian C.
影响因子：
4.2
作者：
PETERSON, JK
通讯作者：
PETERSON, JK
影响因子：
2.3
作者：
Prewein, C;Endemann, M;Wilhelm, E
通讯作者：
Wilhelm, E
影响因子：
3
作者：
Quero, Jose Luis;Villar, Rafael;Poorter, Lourens
通讯作者：
Poorter, Lourens