Response of chondrocyte subpopulations cultured within unloaded and loaded agarose
Response of chondrocyte subpopulations cultured within unloaded and loaded agarose
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DOI：
10.1002/jor.1100160615
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发表时间：
1998-11-01
影响因子：
2.8
通讯作者：
Bader, DL
中科院分区：
文献类型：
--
作者：
Lee, DA;Noguchi, T;Bader, DL
虽然众所周知，软骨细胞的代谢可以通过施加机械应变而改变，但尚不清楚来自软骨浅层和深层的软骨细胞是否以类似的方式做出反应。本研究分别分离牛关节软骨最上层15-20%的软骨细胞（浅层细胞）和剩余组织（深层细胞），并在琼脂糖结构中培养。在培养24小时和72小时后，两个亚群在施加15%静态压缩应变时的细胞变形相同。构建体分别在静态和动态（0.3、1和3 Hz） 15%振幅的应变下孵育。深层细胞的糖胺聚糖合成不受静态应变或3 Hz动态应变的影响，而0.3 Hz的动态应变显著降低糖胺聚糖合成，而1 Hz的动态应变可诱导50%的糖胺聚糖合成刺激（p < 0.001）。表层细胞表现出对糖胺聚糖合成的普遍抑制。动态应变对浅层细胞增殖有促进作用，对深层细胞无影响。先前已经提出，机械转导诱导的糖胺聚糖合成和琼脂糖包埋软骨细胞增殖的控制是不耦合的。本研究中的数据表明，事实上，这两个过程确实发生在全深度细胞分离物中的不同软骨细胞亚群中。
Although it is well known that the metabolism of chondrocytes can be altered by the application of mechanical strain, it is unclear whether chondrocytes from the superficial and deep zones of cartilage respond in a similar manner. In this study, chondrocytes from the uppermost 15-20% (superficial cells) and the remaining tissue (deep cells) from bovine articular cartilage were isolated separately and cultured in agarose constructs. Cell deformation on application of a 15% static compressive strain was identical for both subpopulations after 24 and 72 hours in culture. The constructs were incubated under static and dynamic (0.3, 1, and 3 Hz) strains of 15% amplitude. Glycosaminoglycan synthesis by deep cells was unaffected by static strain or 3 Hz dynamic strain, whereas 0.3 Hz produced a significant reduction and 1 Hz induced a highly significant 50% stimulation of glycosaminoglycan synthesis (p < 0.001). Superficial cells exhibited a general inhibition of glycosaminoglycan synthesis. By contrast, proliferation of superficial cells was stimulated by dynamic strain whereas deep cells were not influenced. It has been suggested previously that mechanotransduction-induced controls of glycosaminoglycan synthesis and proliferation in chondrocytes embedded in agarose are uncoupled. Data presented in this study demonstrate that the two processes do, in fact, occur in different subpopulations of chondrocytes within the full-depth cell isolate.