Combined multiple gene genealogies and phenotypic characters differentiate several species previously identified as Botryosphaeria dothidea
Combined multiple gene genealogies and phenotypic characters differentiate several species previously identified as Botryosphaeria dothidea
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DOI：
10.1080/15572536.2005.11833000
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发表时间：
2004-01
期刊：
影响因子：
2.8
通讯作者：
B. Slippers;P. Crous;S. Denman;T. Coutinho;B. Wingfield;M. Wingfield
中科院分区：
文献类型：
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作者：
B. Slippers;P. Crous;S. Denman;T. Coutinho;B. Wingfield;M. Wingfield
葡萄座腔菌（Botryosphaeria dothidea）是木本植物重要病原菌之一。这分类群通常被接受代表一个种复合体，因此它的身份仍然不清楚。以前的研究要么治疗了B。dothidea作为B的有效名称。Ribis和B。berengeriana或认为他们是独立的实体。更令人困惑的是，B两者都没有前型培养物。dothidea或B。里比斯因此，本研究的目的是筛选和表征这些真菌，并指定一组可用于未来研究的参考培养物。为此，使用来自rDNA（ITS 1、5.8S和ITS 2）、β-微管蛋白和EF 1-α基因的形态学、培养和多等位基因DNA序列数据集来充分表征这些物种。发现Botryosphaeria dothidea不同于B。Ribis，而B. berengeriana被保留为以前名称的同义词。此外，还认为七叶树梭球菌是B的无性型。dothidea，而B. ribis新描述为F. Ribis sp. nov.葡萄座腔菌（Botryosphaeria ribis）与B有明显区别。基于β-tubulin和EF 1-α序列数据对parva进行了序列分析。在这项研究中使用的三个基因区域的组合的系统发育也表明，属葡萄座腔菌代表两个不同的系统发育组合，对应于种与双孢和Fusicoccum anamorphs。
Botryosphaeria dothidea is one of the most commonly reported species in a genus of important pathogens of woody plants. This taxon generally is accepted to represent a species complex, and hence its identity remains unclear. Previous studies either have treated B. dothidea as the valid name for B. ribis and B. berengeriana or argued for them to be separate entities. To add to the confusion, no ex-type cultures are available for either B. dothidea or B. ribis. The aim of the present study, therefore, was to recollect and characterize these fungi and designate a set of reference cultures that can be used in future studies. To this end, morphological, cultural and multi-allelic DNA sequence datasets from the rDNA (ITS 1, 5.8S, and ITS 2), β-tubulin and EF1-α genes were used to fully characterize these species. Botryosphaeria dothidea was found to be distinct from B. ribis, while B. berengeriana was retained as synonym of the former name. Furthermore, Fusicoccum aesculi is accepted as anamorph of B. dothidea, while the anamorph of B. ribis is newly described as F. ribis sp. nov. Botryosphaeria ribis could be distinguished from B. parva based on β-tubulin and EF1-α sequence data. A combined phylogeny of the three gene regions used in this study also showed that the genus Botryosphaeria represents two distinct phylogenetic assemblages that correspond to species with Diplodia and Fusicoccum anamorphs.