Golgin-84 is a Rab1 binding partner involved in Golgi structure
Golgin-84 is a Rab1 binding partner involved in Golgi structure
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DOI：
10.1034/j.1600-0854.2003.00103.x
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发表时间：
2003-03-01
期刊：
影响因子：
4.5
通讯作者：
Warren, G
中科院分区：
文献类型：
--
作者：
Satoh, A;Wang, Y;Warren, G
卷曲螺旋蛋白质的高尔基体家族的成员已经被牵连在囊泡与高尔基体膜和脑池膜彼此的拴系中。许多还结合到rab GTP酶。Golgin-84是一种膜锚定的Golgin，我们现在显示它优先与rab 1 GTP酶的GTP形式结合。通过免疫电镜观察，它也存在于整个高尔基体堆栈中。高尔基体蛋白-84的抗体抑制池膜的堆积在无细胞测定高尔基体重组，而高尔基体蛋白-84的胞质结构域刺激堆积，并增加重新组装的堆栈的长度。高尔基体蛋白-84在NRK细胞中的瞬时表达有助于防止通常由布雷菲德菌素A处理引发的高尔基体解体。总之，这些数据表明，高尔基体-84参与产生和维持高尔基体的架构。
Members of the golgin family of coiled-coil proteins have been implicated in the tethering of vesicles to Golgi membranes and cisternal membranes to each other. Many also bind to rab GTPases. Golgin-84 is a membrane-anchored golgin that we now show binds preferentially to the GTP form of the rab1 GTPase. It is also present throughout the Golgi stack by immuno-EM. Antibodies to golgin-84 inhibit stacking of cisternal membranes in a cell-free assay for Golgi reassembly, whereas the cytoplasmic domain of golgin-84 stimulates stacking and increases the length of re-assembled stacks. Transient expression of golgin-84 in NRK cells helps prevent the disassembly of the Golgi apparatus normally triggered by treatment with brefeldin A. Together these data suggest that golgin-84 is involved in generating and maintaining the architecture of the Golgi apparatus.