Structure and Development of the Herpes-Type Virus of Marek's Disease
Structure and Development of the Herpes-Type Virus of Marek's Disease
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马立克氏病疱疹型病毒的结构和发育
DOI：
10.1093/jnci/41.3.805
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发表时间：
1968
期刊：
影响因子：
--
通讯作者：
P. Biggs
中科院分区：
文献类型：
--
作者：
M. Epstein;B. G. Achong;A. E. Churchill;P. Biggs
用电子显微镜检查感染八种不同马立克氏病菌株的鸡肾培养物中的病毒。培养的细胞本质上是上皮细胞，各毒株感染后均发现典型的疱疹型病毒；未接种的对照培养物中不存在病毒。在每种情况下，病毒在形态上都无法区分，细胞核和细胞质中都有六角形的未成熟颗粒；病毒通过在核膜和细胞质液泡中出芽而成熟，形成更大的成熟颗粒。在阴性对照制剂中，未成熟颗粒的衣壳结构和表面构型也是疱疹病毒组的典型特征。病毒感染的细胞表现出特征性的细胞病理学变化，包括改变的纺锤体小管束。相对于与伯基特淋巴瘤相关的疱疹型 EB 病毒，讨论了疱疹型病毒在动物淋巴瘤中的重要性。
Chicken kidney cultures infected with eight different strains of Marek's disease were investigated with the electron microscope for virus. The cultured cells were epithelial in nature and a typical herpes-type virus was found after infection with each strain; no virus was present in uninoculated control cultures. In each case the virus was morphologically indistinguishable, with hexagonal immature particles in both the nucleus and cytoplasm; the virus matured by budding at the nuclear envelope and cytoplasmic vacuoles to form larger mature particles. In negative-contrast preparations the capsid structure and surface configuration of the immature particles were also typical of the herpes group of viruses. Virus-infected cells showed characteristic cytopathological changes including sheaves of altered spindle tubules. The significance of a herpes-type virus in an animal lymphoma is discussed relative to the herpes-type EB virus associated with the Burkitt lymphoma.