ORIGIN, KINETICS OF CIRCULATION AND FATE INVIVO OF THE MAJOR EXCRETORY SECRETORY PRODUCT OF ACANTHOCHEILONEMA-VITEAE
ORIGIN, KINETICS OF CIRCULATION AND FATE INVIVO OF THE MAJOR EXCRETORY SECRETORY PRODUCT OF ACANTHOCHEILONEMA-VITEAE
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DOI：
10.1017/s0031182000058686
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发表时间：
1989-10-01
期刊：
影响因子：
2.4
通讯作者：
PARKHOUSE, RME
中科院分区：
文献类型：
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作者：
HARNETT, W;WORMS, MJ;PARKHOUSE, RME
葡萄棘唇线虫的分泌物-分泌物（E-S）主要由一种产物组成，分子量为 62 kDa。该分子是在寄生虫生命周期的脊椎动物阶段合成的，并且首先在 L4 寄生虫的 E-S 中检测到。由于具有磷酸胆碱 (PC) 部分，它与人类丝虫寄生虫的 E-S 发生交叉反应。 62 kDa 分子已被用作研究丝虫 E-S 起源和命运的模型。免疫组织学分析表明该分子主要位于寄生虫肠道中。将成年雌性 [35S] 蛋氨酸脉冲的蠕虫移植到未感染的沙鼠中，通过 SDS-PAGE/免疫沉淀分析，4 小时内可在血流中检测到放射性标记的分泌型 62 kDa 抗原。通过在幼稚和受感染的沙鼠宿主中测量注射的纯化 125I 标记材料的清除率来估计分子的全身半衰期。在感染 4-10 周的啮齿动物中，该时间从初始动物的 2-7 小时减少到不到 30 分钟，这一发现与感染组中抗体清除抗原相关。在感染时间较长的动物中，血清半衰期恢复到幼鼠沙鼠中观察到的值。讨论了以下观点：随着感染的进展，半衰期的这种变化可能反映了含有循环免疫复合物的 62 kDa 抗原的性质差异。 125I标记的抗原主要通过肝脏从循环中去除，并最终以非抗原形式从尿液中排出。这项工作首次描述了特定丝虫 E-S 产品的起源、循环动力学和命运，并可能有助于确定带有 PC 的 E-S 成分的功能和评估其诊断效用。
The excretions-secretions (E-S) of Acanthocheilonema viteae consist mainly of one product, molecular weight 62 kDa. This molecule is synthesized during the vertebrate phase of the parasite life-cycle and is first detectable in the E-S of L4 parasites. It is cross-reactive with E-S of human filarial parasites as a consequence of possessing a phosphorylcholine (PC) moiety. The 62 kDa molecule has been employed as a model for the study of the origin and fate of filarial E-S. Immunohistological analysis has shown the molecule to be located predominantly in the parasite gut. Transplantation of adult female [35S] methionine pulsed worms into uninfected jirds resulted in the radio-labelled secreted 62 kDa antigen being detected in the bloodstream within 4 h by SDS-PAGE/immunoprecipitation analysis. The systemic half-life of the molecule as estimated by clearance of injected, purified 125I-labelled material was measured in naive and infected jird hosts. It was reduced from 2-7 h in naive animals to less than 30 min in 4-10 week infected rodents, a finding which correlated with clearance of antigen by antibody in the infected group. In animals infected for longer time periods the serum half-life returned to the values observed in naive jirds. The idea that this change in half-life may reflect differences in the nature of 62 kDa antigen containing circulating immune complexes as infection progresses is discussed. The 125I-labelled antigen is predominantly removed from the circulation via the liver and ultimately excreted in the urine in a non-antigenic form. This work provides the first description of the origin, kinetics of circulation and fate of a defined filarial E-S product and may aid in determining the function and assessing the diagnostic utility of PC-bearing E-S components.