Quantitation of endogenous liver apolipoprotein B mRNA editing.
Quantitation of endogenous liver apolipoprotein B mRNA editing.
复制标题
内源性肝脏载脂蛋白 B mRNA 编辑的定量。
DOI：
10.1016/0006-291x(90)92121-f
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发表时间：
1990
影响因子：
3.1
通讯作者：
Smith,HC
中科院分区：
文献类型：
--
作者：
Backus,JW;Eagleton,MJ;Harris,SG;Sparks,CE;Sparks,JD;Smith,HC
载脂蛋白B的mRNA被翻译成高分子量（apo BH）或低分子量（apo BL）形式的蛋白质，这取决于称为RNA编辑的RNA加工的新形式。载脂蛋白BHmRNA编辑是组织特异性的和经尿道调节的，并且涉及在密码子2153处胞苷向尿苷的转变，从而将谷氨酰胺密码子（CAA）转化为翻译终止密码子（UAA）。比较了3种定量肝脏apo B mRNA编辑内源性水平的方法：（1）Southern印迹杂交结合区分性热洗，（2）竞争体杂交结合区分性热洗和（3）竞争体-聚合酶链反应（竞争体-PCR）。这些数据表明，杂交和PCR可以产生类似的定量时，使用竞争性寡核苷酸。基于竞争体-PCR，提出正常大鼠肝脏和小肠apo B mRNA分别有40%和85%被编辑。
The mRNA for apolipoprotein B is translated into either a high molecular weight (apo BH) or low molecular weight (apo BL) form of the protein depending on a novel form of RNA processing known as RNA editing. Apo BHmRNA editing is both tissue-specific and hormonally regulated and involves transition of cytidine to uridine at codon 2153 thereby converting a glutamine codon (CAA) to a translational stop codon (UAA). Three methods for quantitating the endogenous levels of liver apo B mRNA editing were compared: (1) Southern blot hybridization with discriminative thermal washes, (2) competimer-hybridization with discriminative thermal washes and (3) competimer-polymerase chain reaction (competimer-PCR). The data suggest that hybridization and PCR can yield similar quantitation when competing oligonucleotides are used. Based on competimer-PCR it is proposed that 40% and 85% of normal rat liver and small intestine apo B mRNA (respectively) are edited.