Microscopic overview of crinoid regeneration
Microscopic overview of crinoid regeneration
复制标题
海百合再生的微观概述
DOI：
10.1002/jemt.1187
复制
发表时间：
2001
影响因子：
2.5
通讯作者：
F. Bonasoro
中科院分区：
文献类型：
--
作者：
M. C. Candia Carnevali;F. Bonasoro
海百合以其惊人的再生潜力而闻名，可以迅速而完全地再生自残或创伤性截肢后失去的手臂。为从宏观到分子水平研究再生过程提供了有价值的实验模型。在过去的几年里，我们详细地研究了珊瑚手臂再生的整个过程。总的来说，这种现象可以被描述为一种典型的胚泡再生过程，在这种过程中，新的结构是在假定的调节因子存在的情况下，从迁移的多潜能、活跃的增殖细胞发展而来的。整个过程可分为三个主要阶段：修复阶段、早期再生阶段和高级再生阶段，这些阶段的关键方面与共同的基本机制有关，如细胞迁移和增殖、干细胞和/或去分化细胞的干预、假定的生长因子的贡献，特别是特定的神经源性因子，以及模式形成的机制。本文着重阐述了这一现象的主要方面，并简要介绍了最新的相关研究结果。我们的方法使用了经典的光镜和电子显微镜、免疫细胞化学和组织荧光等方法，对在显著不同的实验条件下获得的标准或异常类型的实验诱导的手臂再生进行了研究，包括极端残缺(外植体)或暴露在假雌激素环境污染中。Microsc.技术资源。55：403-426,2001。©2001 Wiley-Liss公司
Crinoids are well known for their striking regenerative potential and can rapidly and completely regenerate arms lost following self‐induced or traumatic amputation. Thus they provide a valuable experimental model for investigation of the regenerative process from the macroscopic to the molecular level. In these last years we have studied in detail the overall process of arm regeneration in the comatulid Antedon mediterranea. This phenomenon can be described on the whole as a typical blastemal regeneration in which new structures develop from migratory pluripotential, actively proliferating cells in the presence of presumptive regulatory factors. The overall process can be subdivided into three main phases: a repair phase, an early regenerative phase, and an advanced regenerative phase, whose crucial aspects are related to common fundamental mechanisms such as cell migration and proliferation, intervention of stem cells and/or dedifferentiated cells, contribution of putative growth factors, particularly in terms of specific neurally derived factors, and mechanisms of pattern formation. This article focuses on the main aspects of the phenomenon and gives a brief account of the most recent and relevant results. Our approach employs classical methods of light (LM) and electron (TEM and SEM) microscopy, immunocytochemistry, and histofluorescence on experimentally induced arm regenerations of standard or abnormal type obtained in significantly different experimental conditions, including extreme mutilations (explants) or exposure to pseudo‐estrogenic environmental contamination. Microsc. Res. Tech. 55:403–426, 2001. © 2001 Wiley‐Liss, Inc.