ANIMALS AND FUNGI ARE EACH OTHERS CLOSEST RELATIVES - CONGRUENT EVIDENCE FROM MULTIPLE PROTEINS
ANIMALS AND FUNGI ARE EACH OTHERS CLOSEST RELATIVES - CONGRUENT EVIDENCE FROM MULTIPLE PROTEINS
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DOI：
10.1073/pnas.90.24.11558
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发表时间：
1993-12-15
影响因子：
11.1
通讯作者：
PALMER, JD
中科院分区：
文献类型：
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作者：
BALDAUF, SL;PALMER, JD
利用25种蛋白质的序列分析了植物、动物和真菌之间的系统发育关系。发现了四个插入/缺失，这四个插入/缺失由所讨论的三个分类组中的两个共享，并且所有四个都是动物和真菌相对于植物、原生动物和细菌的唯一共享。其中包括在翻译延伸因子1pha中插入一个12个氨基酸，以及在烯醇化酶中有三个小缺口。最大简约树是根据已公布的25种蛋白质中测序最广泛的四种蛋白质的数据构建的，这四种蛋白质是肌动蛋白、α-微管蛋白、β-微管蛋白和延伸因子1α，后者由三个新的外群序列补充。所有这四种蛋白质都将动物和真菌作为一个单一的类群放在一起，排除了植物和广泛多样性的原生生物。在所有情况下，Bootstrap分析都不支持动物-植物或真菌-植物分支。与传统和当前的分类不同，多条证据之间的这种一致性强烈表明，动物和真菌是姐妹群，而植物构成了独立的进化谱系。
Phylogenetic relationships among plants, animals, and fungi were examined by using sequences from 25 proteins. Four insertions/deletions were found that are shared by two of the three taxonomic groups in question, and all four are uniquely shared by animals and fungi relative to plants, protists, and bacteria. These include a 12-amino acid insertion in translation elongation factor 1alpha and three small gaps in enolase. Maximum-parsimony trees were constructed from published data for four of the most broadly sequenced of the 25 proteins, actin, alpha-tubulin, beta-tubulin, and elongation factor 1alpha, with the latter supplemented by three new outgroup sequences. All four proteins place animals and fungi together as a monophyletic group to the exclusion of plants and a broad diversity of protists. In all cases, bootstrap analyses show no support for either an animal-plant or fungal-plant clade. This congruence among multiple lines of evidence strongly suggests, in contrast to traditional and current classification, that animals and fungi are sister groups while plants constitute an independent evolutionary lineage.