Antioxidant Protection by American Ginseng in Pancreatic β-Cells
Antioxidant Protection by American Ginseng in Pancreatic β-Cells
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DOI：
10.1142/s0192415x08006399
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发表时间：
2008-01-01
影响因子：
5.7
通讯作者：
Yuan, Chun-Su
中科院分区：
文献类型：
--
作者：
Lin, Elaine;Wang, Yong;Yuan, Chun-Su
糖尿病条件下的高血糖可能导致胰腺β细胞的氧化应激。导致其功能障碍和外周组织内的胰岛素抗性。以前的研究表明，西洋参浆果提取物可能具有降血糖作用。并提供抗氧化保护。我们研究了西洋参浆果提取物和西洋参Re在胰腺β细胞系中的作用。MINA以确定这两个属性是否相关。通过过氧化氢孵育将细胞暴露于氧化应激，并通过2 '，7'-二氯荧光素二乙酸酯的氧化来测量氧化应激。这些细胞对100-500 μ M的过氧化氢表现出浓度相关的反应。在用提取物处理细胞10分钟的急性条件下，我们观察到氧化损伤减少，表明直接清除作用。将细胞与浸提液长期孵育48小时也证明了氧化应激的减弱。在高浓度下，Re在MIN-6细胞中显示出温和的抗氧化作用。我们的胰岛素释放观察还表明，提取物可能有助于增加细胞的胰岛素分泌。我们的数据表明，观察到的人参降低血糖水平的能力可能与其对胰腺β细胞的抗氧化作用有关。
Hyperglycemia in diabetic conditions May cause oxidative stress in Pancreatic beta-cells. leading to their dysfunction and insulin resistance within peripheral tissues. Previous Studies suggest that American ginseng berry extract may have hypoglycemic effects. as well as offer antioxidant protection. We examined effects of American ginseng berry extract and ginsenoside Re in a pancreatic beta-cell line. MINA to determine if these two properties are related. Cells were exposed to oxidative stress via hydrogen peroxide incubation and oxidative stress was measured by oxidation of 2',7'-dichlorofluorescin diacetate. These cells showed a concentration-related response to hydrogen peroxide at 100-500 mu M. In acute conditions where cells were treated with the extract for 10min, we observed reduced oxidant injury suggesting direct scavenging effects. Chronic incubation of cells with the extract for 48 hours also demonstrated attenuation of oxidative stress. At high concentrations, Re showed a mild antioxidant effect in MIN-6 cells. Our insulin release observations also showed that the extract may help to increase insulin secretions from the cells. Our data suggest that the observed ability of Ginseng to reduce blood glucose levels may be linked to its antioxidant effects on Pancreatic beta-cells.