Bio-refinery of waste streams for green and efficient succinic acid production by engineered Yarrowia lipolytica without pH control
Bio-refinery of waste streams for green and efficient succinic acid production by engineered Yarrowia lipolytica without pH control
复制标题
废物流生物精炼，通过工程解脂耶氏酵母绿色高效生产琥珀酸，无需 pH 控制
DOI：
10.1016/j.cej.2019.04.092
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发表时间：
2019-09-01
影响因子：
15.1
通讯作者：
Lin, Carol Sze Ki
中科院分区：
文献类型：
--
作者：
Li, Chong;Ong, Khai Lun;Lin, Carol Sze Ki
本文提出了一种高效、绿色和可持续的琥珀酸 (SA) 发酵方法，其中首次使用来自各种废物流的富含葡萄糖的水解产物作为解脂耶氏酵母 (Yarrowia lipolytica) 生产 SA 的底物，无需 pH 控制。首先，我们发现解脂耶氏酵母 PGC202 能够高效消耗富含葡萄糖的混合食物垃圾 (MFW) 水解产物来生产 SA。培养基优化后，以60 g/L含葡萄糖的MFW水解液为碳源，添加3%胰蛋白胨，isFBB发酵得到的SA效价为31.7 g/L，产量为0.52 g/g，生产率为0.60 g/L/h。当使用来自其他废物流的富含葡萄糖的水解产物时，在低pH下通过酵母发酵实现了最高的SA产量为0.61 g/g，这证明了解脂耶氏酵母广泛的底物适应性和巨大的潜力。通过补料浓缩 MFW 水解产物，补料分批发酵的 SA 效价高达 71.6 g/L，pH 自然降低至 2.8。最后，采用改进的回收方法，仅用少量酸化酸即可从酸性发酵液中回收了总SA的68.3%，纯度在89.5%至91.0%之间，这表明低pH条件下SA生产相对于中性条件下生产在经济改善和环境可持续性方面具有巨大优势。总的来说，这项研究克服了未来大规模发酵SA生产的主要经济缺陷之一，同时也说明了生物SA生产的广阔前景。
This paper presents an efficient, green and sustainable approach for succinic acid (SA) fermentation, in which glucose-rich hydrolysates from various waste streams were used as the substrate for SA production by Yarrowia lipolytica without pH control for the first time. First, Y. lipolytica PGC202 was found to be able to consume glucose-rich mixed food waste (MFW) hydrolysate for SA production with high efficiency. After medium optimization, a SA titer at 31.7 g/L with a yield of 0.52 g/g and productivity of 0.60 g/L/h was obtained from isFBB fermentation when using 60 g/L glucose-containing MFW hydrolysate as the carbon source supplemented with 3% tryptone. When glucose-rich hydrolysate from other waste streams was used, the highest SA yield of 0.61 g/g via yeast fermentation at low pH was achieved, which demonstrated the extensive substrate adaptability and great potential of Y. lipolytica. By feeding concentrated MFW hydrolysate, SA titer up to 71.6 g/L was achieved from fed-batch fermentation with pH decreased naturally to 2.8. Finally, with the revised recovery method, 68.3% of the total SA with purity between 89.5% and 91.0% was recovered from the acidic fermentation broth with only low input of acid for acidification, which demonstrated the great advantages of SA production at low pH over that under neutral conditions in terms of economic improvement and environmental sustainability. In general, this study overcomes one of the major economic drawbacks for future feasible large-scale fermentative SA production, and it also illustrates a promising prospective for bio-SA production.