The seasonal development dynamics of the yak hair cycle transcriptome
The seasonal development dynamics of the yak hair cycle transcriptome
复制标题
牦牛毛发周期转录组的季节发育动态
DOI：
10.1186/s12864-020-6725-7
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发表时间：
2020-05-11
期刊：
影响因子：
4.4
通讯作者：
Yan, Ping
中科院分区：
文献类型：
--
作者：
Bao, Pengjia;Luo, Jiayu;Yan, Ping
研究背景哺乳动物毛发在哺乳动物适应气候变化的能力中起着重要作用。牦牛被毛的季节性循环有助于其适应高海拔环境，但其毛囊细胞在发育过程中增殖分化的调控机制尚不清楚。在这里，使用时间序列数据的转录组和激素含量，我们系统地分析了机制调节的周期性表达的牦牛毛发发育和审查如何不同组合的遗传途径调节高频的发展和cycling.ResultsThis研究使用高通量RNA测序提供了一个详细的描述，在15个样本的全球基因表达在牦牛毛发周期的5个发育时间点。根据聚类分析，我们发现这15个样本可以明显地分为三个阶段，代表了头发周期中的不同发育时期。在这3个连续发育时期共鉴定出2316个基因，其表达模式可分为9个簇。在毛发生长期，参与激活毛囊生长的基因高度表达，如WNT途径、FGF途径和一些与毛囊分化相关的基因。在退化期，抑制分化和促进毛囊细胞凋亡的基因高度表达，如BMP 4和Wise。在休止期，抑制毛囊活性的基因高度表达，如DKK 1和BMP 1。通过共表达分析，我们发现了一些与激素高度相关的模块化枢纽基因，如SLF2，BOP1和DPP8。它们可能发挥独特的作用，在激素调节的事件与毛发cycle.ConclusionsOur结果揭示了牦牛的季节性毛发周期的表达模式和分子机制。该研究结果对于进一步了解牦牛的高山适应机制具有重要价值，对于充分利用牦牛毛资源、促进高原牧区经济发展具有重要意义。
BackgroundMammalian hair play an important role in mammals' ability to adapt to changing climatic environments. The seasonal circulation of yak hair helps them adapt to high altitude but the regulation mechanisms of the proliferation and differentiation of hair follicles (HFs) cells during development are still unknown. Here, using time series data for transcriptome and hormone contents, we systematically analyzed the mechanism regulating the periodic expression of hair development in the yak and reviewed how different combinations of genetic pathways regulate HFs development and cycling.ResultsThis study used high-throughput RNA sequencing to provide a detailed description of global gene expression in 15 samples from five developmental time points during the yak hair cycle. According to clustering analysis, we found that these 15 samples could be significantly grouped into three phases, which represent different developmental periods in the hair cycle. A total of 2316 genes were identified in these three consecutive developmental periods and their expression patterns could be divided into 9 clusters. In the anagen, genes involved in activating hair follicle growth are highly expressed, such as the WNT pathway, FGF pathway, and some genes related to hair follicle differentiation. In the catagen, genes that inhibit differentiation and promote hair follicle cell apoptosis are highly expressed, such as BMP4, and Wise. In the telogen, genes that inhibit hair follicle activity are highly expressed, such as DKK1 and BMP1. Through co-expression analysis, we revealed a number of modular hub genes highly associated with hormones, such as SLF2, BOP1 and DPP8. They may play unique roles in hormonal regulation of events associated with the hair cycle.ConclusionsOur results revealed the expression pattern and molecular mechanisms of the seasonal hair cycle in the yak. The findings will be valuable in further understanding the alpine adaptation mechanism in the yak, which is important in order to make full use of yak hair resources and promote the economic development of pastoral plateau areas.