(7,8,9/10) Differential expression analysis throughout the weaning period in the mouse cerebral cortex
(7,8,9/10) Differential expression analysis throughout the weaning period in the mouse cerebral cortex
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(7,8,9/10)小鼠大脑皮层整个断奶期的差异表达分析
DOI：
10.1016/j.bbrc.2012.12.150
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
H.
中科院分区：
文献类型：
--
作者：
Maeda;N.;Okada;S.;Abe;K.;Aizawa;H.
哺乳动物在断奶时，从喝母乳转变为吃环境来源的食物。这些食物含有天然化合物，具有新的味道和质地，在母乳喂养结束后首次提供给年轻人。这种新的进食体验可能会改变哺乳动物婴儿的认知大脑功能，增加他们对食物环境的反应。由于大脑皮层是认知和学习的中枢器官，我们使用微阵列分析研究了断奶前后小鼠大脑皮层全基因表达谱的差异。在45，037个小鼠基因中，35个基因上调，31个基因下调，以响应断奶。特别是，立即早期基因，分子伴侣和髓鞘相关基因上调。原位杂交分析表明，即时早期基因，Egr-2/KROX-20的mRNA，从细胞核运输到细胞体在5/6层的躯体感觉皮层在断奶。相比之下，在没有任何食物供应的动物，除了母乳，Egr-2/KROX-20 mRNA保留在躯体感觉皮层的核内。这些数据表明，新的经验，食物摄入调节基因表达谱在小鼠大脑皮层在断奶阶段。
At weaning, mammals switch from drinking mother’s milk to eating foods of environmental origin. These foods contain natural compounds with novel tastes and textures, which are provided to the young for the first time following the termination of breastfeeding. This novel eating experience may alter the cognitive brain function of mammalian babies, increasing their reactions to their food environments. Because the cerebral cortex is a central organ for cognition and learning, we investigated differences in whole-gene expression profiles in the mouse cerebral cortex using microarray analysis before and after weaning. Of 45,037 murine genes, 35 genes were upregulated and 31 genes were downregulated, in response to weaning. In particular, immediate early genes, molecular chaperones, and myelin-related genes were upregulated. In situ hybridization analysis revealed that the mRNA for an immediate early gene, Egr-2/KROX-20, was transported from the nucleus to the cell body at layer 5/6 of the somatosensory cortex during weaning. In contrast, in animals without any food supply other than mother’s milk, Egr-2/KROX-20 mRNA was retained within the nucleus at the somatosensory cortex. These data suggest that the novel experience of food intake modulates gene expression profiles in the murine cerebral cortex at the weaning stage.