The chemical nature of hœmochromogen and its carbon monoxide compound.
The chemical nature of hœmochromogen and its carbon monoxide compound.
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DOI：
10.1098/rspb.1926.0064
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发表时间：
1926-12-01
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影响因子：
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通讯作者：
Hill, R
中科院分区：
文献类型：
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作者：
Hill, R
铁-血卟啉化合物在还原时显示出类似还原血红素的吸收光谱，在加入珠蛋白、吡啶或氨时，产生血红色原。在将以N计为0.0316 N的吡啶溶液加入到以Fe计为0.0316 N的血红素溶液中时，发现在吡啶不足的情况下，观察到还原的血红素光谱以及吡啶-血色原光谱。当加入2当量吡啶时，仅得到血色原光谱，再加入吡啶则无变化。观察到的CO从还原血红素的CO化合物中的演化与理论计算的1分子CO与血红素的组合一致，无论是在不存在还是存在吡啶的情况下。含有不同量吡啶的血红素溶液在CO存在下还原，并测量[α]带的位置。在有限量的CO存在下，a带首先向红色移动，当加入1当量吡啶时获得最小阅读。随着吡啶的进一步加入，[alpha]带再次移向紫色。随着CO的无限供应，谱带移到红色，然后保持相当恒定。作者提出了一氧化碳血红蛋白原和血红蛋白原的计算公式。他认为还原型血红素是去掉了辅助物质的血红蛋白的色素，相当于安森和米尔斯基的“血红素”。
Fe-hematoporphyrin compound shows on reduction an absorption spectrum like reduced hematin, and on addition of globin, pyridine or ammonia, gave hemo-chromogen. On addition of a pyridine solution, 0.0316 N in terms of N, to a hematin solution, 0.0316 N in terms of Fe, it was found that in the presence of insufficient pyridine a reduced hematin spectrum is seen together with a pyridine-hemochromogen spectrum. When 2 equivalents of pyridine are added only the hemochro-mogen spectrum is obtained, and further addition of pyridine causes no change. The observed evolution of CO from the CO compound of reduced hematin agreed with the theoretical calculated for 1 molecule of CO in combination with the hematin, both in the absence and presence of pyridine. Solutions of hematin containing different amounts of pyridine were reduced in the presence of CO and the position of the [alpha] band measured. With a limited amount of CO available the a band is first shifted to the red, a minimum reading being obtained when 1 equivalent of pyridine has been added. With further additions of pyridine the [alpha] band again shifts to the violet. With unlimited supply of CO the band shifts to the red, and then remains fairly constant. The author proposes formulae for CO hemo-chromogen and hemocnromogen. He regards reduced hematin as the pigment of hemoglobin shorn of accessory substances, and corresponding to the "haem" of Anson and Mirsky.