Comparison of label-free and GFP multiphoton imaging of hair follicle-associated pluripotent (HAP) stem cells in mouse whiskers.
Comparison of label-free and GFP multiphoton imaging of hair follicle-associated pluripotent (HAP) stem cells in mouse whiskers.
复制标题
小鼠胡须中毛囊相关多能 (HAP) 干细胞的无标记和 GFP 多光子成像的比较。
DOI：
10.1080/15384101.2015.1090064
复制
发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Koenig,Karsten
中科院分区：
文献类型：
--
作者：
Uchugonova,Aisada;Cao,Wenluo;Hoffman,RobertM;Koenig,Karsten
毛囊相关多能干细胞(HAP)可以分化为多种细胞类型，包括神经元和心肌细胞，并已被证明可以修复小鼠的周围神经和脊髓。可以从每个患者身上获得HAP干细胞，用于再生医学，克服了免疫排斥的问题。此前，我们已经证明了转基因小鼠中的GFP等遗传编码的蛋白质标记物可以通过多光子断层扫描在活体中显示HAP干细胞。在没有GFP等外源标记的情况下，活体干细胞的检测和可视化对于人类的应用将是重要的。在本报告中，我们展示了由于NAD(P)H/黄素等内在荧光团的缘故，通过多光子断层扫描可以无标记地显示小鼠胡须中的毛囊干细胞。我们在分离的小鼠胡须中比较了GFP标记的HAP干细胞和未标记的干细胞的多光子断层扫描。我们表明，用无标记多光子断层摄影术观察HAP干细胞与使用GFP标记的干细胞检测是相似的。本文所述结果对检测和分离用于再生医学的人HAP干细胞具有重要意义。
Hair-follicle-associated pluripotent (HAP) stem cells can differentiate into many cell types, including neurons and heart muscle cells, and have been shown to repair peripheral nerves and the spinal cord in mice. HAP stem cells can be obtained from each individual patient for regenerative medicine which overcomes problems with immune rejection. Previously, we have demonstrated that genetically-encoded protein markers such as GFP in transgenic mice can be used to visualize HAP stem cellsin vivoby multiphoton tomography. Detection and visualization of stem cellsin vivowithout exogenous labels such as GFP would be important for human application. In the present report, we demonstrate label-free visualization of hair follicle stem cells in mouse whiskers by multiphoton tomography due to the intrinsic fluorophores such as NAD(P)H/flavins. We compared multiphoton tomography of GFP-labeled HAP stem cells and unlabeled stem cells in isolated mouse whiskers. We show that observation of HAP stem cells by label-free multiphoton tomography is comparable to detection using GFP-labeled stem cells. The results described here have important implications for detection and isolation of human HAP stem cells for regenerative medicine.