Glucose starvation alters lipid-linked oligosaccharide biosynthesis in Chinese hamster ovary cells.
Glucose starvation alters lipid-linked oligosaccharide biosynthesis in Chinese hamster ovary cells.
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葡萄糖饥饿改变了中国仓鼠卵巢细胞中脂联寡糖的生物合成。
DOI：
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发表时间：
1981
期刊：
影响因子：
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通讯作者：
S. Kornfeld
中科院分区：
文献类型：
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作者：
J. Rearick;A. Chapman;S. Kornfeld
本文研究了缺糖对中国仓鼠卵巢细胞合成脂联寡糖的影响。当将这些细胞置于不含葡萄糖的无血清Dulbecco最小必需培养基中时，通常的Glc3Man9GlcNAc2脂质连接寡糖的合成迅速停止，并积累了组成为Man9GlcNAc2的较小种类。后一种化合物被葡糖基化并转移到蛋白质中，随后在蛋白质中进行加工。这些发现表明，中国仓鼠卵巢细胞在缺乏葡萄糖时利用替代糖基化途径。
The effect of glucose deprivation on the synthesis of lipid-linked oligosaccharides by Chinese hamster ovary cells has been studied. When these cells are placed in serum-free Dulbecco's minimal essential medium devoid of glucose, there is a rapid cessation of the synthesis of the usual Glc3Man9GlcNAc2 lipid-linked oligosaccharide and the accumulation of a smaller species with the composition Man9GlcNAc2. This latter compound is the glucosylated and transferred to protein where it is subsequently processed. These findings demonstrate that Chinese hamster ovary cells utilize an alternate glycosylation pathway when deprived of glucose.