Arabidopsis cryptochrome is responsive to Radiofrequency (RF) electromagnetic fields
Arabidopsis cryptochrome is responsive to Radiofrequency (RF) electromagnetic fields
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DOI：
10.1038/s41598-020-67165-5
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发表时间：
2020-07-09
影响因子：
4.6
通讯作者：
Martino, Carlos F.
中科院分区：
文献类型：
--
作者：
Albaqami, Maria;Hammad, Merfat;Martino, Carlos F.
生命系统如何对弱电磁场做出反应是感觉生物学中尚未解决的主要挑战之一。最近的证据表明隐花色素，一种进化上保守的黄素蛋白受体，在从植物到候鸟的生物体的磁场响应中起作用。然而，隐花色素是否满足作为生物磁传感器的标准仍有待确定。目前，化学磁感受的基本机制的理论预测已经得到实验观察的支持，暴露于MHz范围内的射频（RF）干扰鸟类的方向和哺乳动物细胞呼吸。在这里，我们表明，在保持一定的量子物理假设，一个弱的7MHz的射频磁场显着降低生物响应蓝光的隐花色素受体cry1在拟南芥幼苗。使用体内磷酸化试验，专门检测激活隐花色素，我们证明，RF暴露减少与生物活性相关的构象变化。此外，RF暴露改变隐花色素依赖的植物生长反应和基因表达的理论预测一致的程度。据我们所知，这是第一次证明的生物受体响应RF暴露，提供重要的新的影响，磁传感以及未来可能的应用在生物技术和医学。
How living systems respond to weak electromagnetic fields represents one of the major unsolved challenges in sensory biology. Recent evidence has implicated cryptochrome, an evolutionarily conserved flavoprotein receptor, in magnetic field responses of organisms ranging from plants to migratory birds. However, whether cryptochromes fulfill the criteria to function as biological magnetosensors remains to be established. Currently, theoretical predictions on the underlying mechanism of chemical magnetoreception have been supported by experimental observations that exposure to radiofrequency (RF) in the MHz range disrupt bird orientation and mammalian cellular respiration. Here we show that, in keeping with certain quantum physical hypotheses, a weak 7MHz radiofrequency magnetic field significantly reduces the biological responsivity to blue light of the cryptochrome receptor cry1 in Arabidopsis seedlings. Using an in vivo phosphorylation assay that specifically detects activated cryptochrome, we demonstrate that RF exposure reduces conformational changes associated with biological activity. RF exposure furthermore alters cryptochrome-dependent plant growth responses and gene expression to a degree consistent with theoretical predictions. To our knowledge this represents the first demonstration of a biological receptor responding to RF exposure, providing important new implications for magnetosensing as well as possible future applications in biotechnology and medicine.