Enhancement of hydroxyapatite-mediated three-dimensional-like proliferation of mouse fibroblasts by heat treatment: Effects of heat shock-induced p38 MAPK pathway
Enhancement of hydroxyapatite-mediated three-dimensional-like proliferation of mouse fibroblasts by heat treatment: Effects of heat shock-induced p38 MAPK pathway
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DOI：
10.1002/jbm.a.30362
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发表时间：
2005-09-15
影响因子：
4.9
通讯作者：
Kano, Y
中科院分区：
文献类型：
--
作者：
Hiragami, F;Akiyama, J;Kano, Y
复合型羟基磷灰石（HA）与细胞的生物相容性受多种环境因素的影响。本研究的目的是确定p38丝裂原活化蛋白激酶（MAPK）通路是否在热处理后HA介导的小鼠成纤维细胞三维（3D）样增殖的增强中起关键作用。在44 ℃下热处理5、10、20和30 min后，将C3 H10 T1/2小鼠成纤维细胞与HA颗粒一起培养10周。热处理20 min的细胞形成3D样增殖模式的平均速率仅为未处理细胞的2.1倍，但热处理5分钟和10分钟的细胞形成3D样增殖模式的平均速率显著高于未处理细胞（分别高3.7倍和3.3倍）（p < 0.01）。Western blot
Regulation of the biocompatibility of compositional hydroxyapatite (HA) with cells is affected by various environmental factors. The aim of this study was to determine whether the p38 mitogen-activated protein kinase (MAPK) pathway has a key role in enhancement of HA-mediated three-dimensional (3D)-like proliferation of mouse fibroblasts after heat treatment. C3H10T1/2 mouse fibroblasts were cultured with HA granules for 10 weeks after heat treatment at 44 degrees C for 5, 10, 20, and 30 min. The mean rate of formation of 3D-like proliferation patterns by cells heat treated for 20 min was only 2.1-fold higher than that by untreated cells, but the mean rates of formation of 3D-like proliferation patterns by cells heat treated for 5 and 10 min were significantly higher (3.7- and 3.3-fold higher, respectively) than that by untreated cells (p < 0.01). Western blot