Application of an Analog of 9, 10-ketol-octadecadienoic acid (KODA), Affected Flower Bud Formation and MdTFL1 and MdFT1 Gene Expressions in Apple Buds under Heavy-crop and Shade Conditions
Application of an Analog of 9, 10-ketol-octadecadienoic acid (KODA), Affected Flower Bud Formation and MdTFL1 and MdFT1 Gene Expressions in Apple Buds under Heavy-crop and Shade Conditions
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DOI：
10.21273/jashs.138.2.102
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发表时间：
2013-03-01
影响因子：
1.9
通讯作者：
Watanabe, Naoharu
中科院分区：
文献类型：
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作者：
Kittikorn, Monrudee;Okawa, Katsuya;Watanabe, Naoharu
以苹果为试材，研究了9-脂氧合酶催化亚麻酸合成内源9，10-酮醇-十八烯酸(Koda)含量的变化。米勒。瓦尔家蝇(Borkh.)Mansf.]巴兹。此外，还研究了重茬处理和遮荫条件下，15，16-氯，羟基-9-羟基-10-氧代-12(Z)-十八碳烯酸(CKoda)对苹果花芽形成和MdTFL1(终花I)和MdFT1(开花基因t1)基因表达的影响。芽中内源Koda含量的增加。疏果处理的花芽形成率在盛花后63d高于对照，而对照没有增加。在遮荫处理和重茬树中，CKoda处理的花蕾中MdTFl1的表达低于未处理的花蕾。相反，在遮荫条件下，CKoda处理的芽中MdFT1的表达高于未处理的芽。这些结果表明，内源Koda可能与花芽的形成有关，Koda的应用可能通过影响MdTFl1和MdFT1而有效地提高花芽形成的比例。
Changes of endogenous 9, 10-ketol-octadecadienoic acid (KODA) concentrations, which is synthesized from linolenic acid by 9-lipoxygenase, were analyzed in apple [Malus xsylvestris (L.) Mill. var. domestica (Borkh.) Mansf.] buds. In addition, the effects of 15, 16-chloro, hydroxy-9-hydroxy-10-oxo-12(Z)-octadecenoic acid (CKODA) application, which is an analog of KODA, on flower bud formation and the expression of MdTFL1 (terminal flower I) and MdFT1 (flowering locus t 1) genes in apple buds were investigated in heavy-crop treatment (HCT) and under shade. An increase of endogenous KODA in the buds. in the fruit-thinning treatment, which resulted in a higher proportion of flower bud formation than in HCT, was observed at 63 days after full bloom, but no such increase was found in HCT. In the shade-treated and heavy-crop trees, the expression of MdTFL1 in the buds to which CKODA was applied was lower than that in untreated buds. In contrast, under shade, the expression of MdFT1 in the CKODA-treated buds was higher than that in untreated buds. These results suggest that endogenous KODA may be associated with flower bud formation, and its application may be effective at improving the proportion of flower bud formation through its effect on MdTFL1 and MdFT1.