Effect of a null mutation of the oviduct-specific glycoprotein gene on mouse fertilization
Effect of a null mutation of the oviduct-specific glycoprotein gene on mouse fertilization
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DOI：
10.1042/bj20030466
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发表时间：
2003-09-01
影响因子：
4.1
通讯作者：
Sendai, Y
中科院分区：
文献类型：
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作者：
Araki, Y;Nohara, M;Sendai, Y
哺乳动物的受精过程发生在女性生殖道内复杂的微环境中。输卵管特异性糖蛋白（OGP）是几丁质酶蛋白家族的一员，已在包括人类在内的多种哺乳动物的输卵管液中鉴定出。尽管OGP被广泛认为参与哺乳动物受精过程，包括精子功能和配子相互作用，但基于体外实验结果，其生理意义仍存在争议。本研究建立了OGP基因敲除（ogp（-/-））小鼠模型，并对其生殖特性进行了初步研究，以探讨OGP的生理功能。从使用体内或体外系统的研究中获得的结果表明，ogp（-/-）女性的生育力在正常范围内。这些结果表明，OGP在体内受精过程中不是必需的，至少在小鼠中是如此。
The mammalian fertilization process takes place in a complex microenvironment within the female genital tract. A member of the chitinase protein family, oviduct-specific glycoprotein (OGP), has been identified in oviductal fluid from various mammalian species, including humans. Although OGP is widely believed to be involved in the process of mammalian fertilization, including spermatozoon function and gamete interactions, based on experimental results obtained in vitro, its physiological significance remains controversial. The present study established OGP gene-null (ogp(-/-)) mice, and primarily characterized their reproductive properties to study the physiological function(s) of OGP. Results obtained from studies using an in vivo or in vitro system showed that the fertility of ogp(-/-) females was within normal limits. These results indicate that OGP is not essential for the process of in vivo fertilization, at least in mice.