Hepatitis C virus core region: helper T cell epitopes recognized by BALB/c and C57BL/6 mice.
Hepatitis C virus core region: helper T cell epitopes recognized by BALB/c and C57BL/6 mice.
复制标题
丙型肝炎病毒核心区：BALB/c 和 C57BL/6 小鼠识别的辅助 T 细胞表位。
作者：
K. Kakimi;K. Kuribayashi;M. Iwashiro;T. Masuda;M. Sakai;W. Ling;Y. Kubo;H. Kobayashi;K. Higo;M. Seki
在这项研究中，我们研究了两个品系的小鼠对丙型肝炎病毒核心蛋白亲水性部分的B细胞和T细胞的反应，并确定了各自的抗原决定因素。用重组丙型肝炎病毒核心蛋白和弗氏完全佐剂皮下注射免疫BALB/c(H-2d)和C57BL/6(B6：H-2b)小鼠。用ELISA法测定，BALB/c小鼠的抗体水平始终高于B6小鼠。然而，根据一项使用覆盖丙型肝炎病毒核心蛋白亲水部分的非重叠多肽的实验，每个毒株的血清中的抗体结合到核心蛋白的N端区域，氨基酸范围为1到28个氨基酸(MSTNPKPQRKIKRNTNRRPQDVKFPGGG)。BALB/c小鼠的T细胞对引流淋巴结细胞的体外增殖反应也高于B6小鼠。用重组核心蛋白重复刺激的引流淋巴结细胞的体外有限稀释培养，建立了两个品系小鼠的T细胞克隆，并对其进行了鉴定。这些克隆的表面标志为Thy-1.2+、CD3+、TCRαβ+、CD4+和CD8+。在抗CD4或抗MHC II类单抗的存在下，增殖反应受到抑制。BALB/c小鼠T细胞系识别丙型肝炎病毒核心72~91位氨基酸的表位(EGRAWAQPGYPWPLYGNEGL)。B6小鼠的T细胞系识别55到74个氨基酸的表位(RPQPRGRRQPIPKARQPEGR)。因此，具有不同MHC单倍型的小鼠识别不同的非重叠的丙型肝炎病毒核心蛋白的T细胞抗原决定簇。
In this study, we characterized the B cell and T cell responses to the hydrophilic portion of hepatitis C virus (HCV) core protein in two strains of mice and identified the respective antigen determinants. BALB/c (H-2d) and C57BL/6 (B6:H-2b) mice were immunized by a subcutaneous injection of recombinant HCV core protein together with Freund's complete adjuvant. The level of antibody production, as determined by ELISA, was consistently higher in BALB/c than in B6 mice. However, antibodies in sera from each strain bound to the N-terminal region of the core protein within amino acids 1 to 28 (MSTNPKPQRKIKRNTNRRPQDVKFPGGG), according to an experiment using non-overlapping peptides that covered the hydrophilic portion of HCV core protein. The T cell responses were also higher in BALB/c than in B6 mice with respect to the proliferative responses of the draining lymph node cells in vitro. By limiting dilution cultures of the draining lymph node cells in vitro repetitively stimulated with recombinant core protein, T cell clones were established from both strains of mice and characterized. The surface markers of these clones were Thy-1.2+, CD3+, TCR alpha beta+, CD4+ and CD8+. The proliferative responses were inhibited in the presence of anti-CD4 or anti-MHC class II monoclonal antibodies. The T cell lines in BALB/c mice recognized an epitope in HCV core at amino acids 72 to 91 (EGRAWAQPGYPWPLYGNEGL). The T cell lines in B6 mice recognized an epitope at amino acids 55 to 74 (RPQPRGRRQPIPKARQPEGR). Thus, mice with different MHC haplotypes recognized different non-overlapping T cell antigenic determinants of HCV core proteins.
影响因子：
4.4
作者：
D. Milich;Alan McLachlan;A M Moriarty;G. B. Thornton
通讯作者：
D. Milich;Alan McLachlan;A M Moriarty;G. B. Thornton
影响因子：
56.9
作者：
MORIYAMA, T;GUILHOT, S;CHISARI, FV
通讯作者：
CHISARI, FV