Correlation of oxidant status with oxidative tissue damage in patients with rheumatoid arthritis
Correlation of oxidant status with oxidative tissue damage in patients with rheumatoid arthritis
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DOI：
10.1007/s10067-014-2597-z
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发表时间：
2014-11-01
影响因子：
3.4
通讯作者：
Chatterjee, Mitali
中科院分区：
文献类型：
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作者：
Datta, Suhana;Kundu, Sunanda;Chatterjee, Mitali
类风湿关节炎(RA)是一种衰弱的自身免疫性疾病，其病因尚不清楚，但研究一直表明，过多的炎症机制最终导致慢性对称性和侵蚀性滑膜炎。重要的是，活性氧簇(ROS)被认为是RA明显的破坏性、增生性滑膜炎的直接原因。因此，这项研究旨在确定氧化应激程度和疾病活动评分(DAS28)是否与氧化损伤的下游影响相关。用流式细胞仪检测滑液(SF)来源的中性粒细胞的氧化还原状态，以总ROS和羟基自由基为指标。在分子损伤标志物中，蛋白质羰化和脂质过氧化反应采用分光光度法检测，S-亚硫醇的检测采用荧光法。中性粒细胞是类风湿关节炎患者外周血中的主要细胞成分，其产生的ROS和羟基自由基水平与蛋白质羰化和脂质过氧化密切相关。而氧化损伤指标与DAS28呈正相关。综上所述，在RA患者中，ROS和DAS28水平与氧化损伤标志物之间的强烈相关性表明，氧化应激的测量可以作为监测RA疾病严重程度的生物标志物。
Rheumatoid arthritis (RA) is a debilitating autoimmune disease whose etiology remains unknown, but studies have consistently implicated a plethora of inflammatory mechanisms culminating in chronic symmetric and erosive synovitis. Importantly, reactive oxygen species (ROS) have been attributed to directly contribute towards the destructive, proliferative synovitis evident in RA. Accordingly, this study aimed to establish whether the degree of oxidative stress and disease activity score (DAS28) correlated with the downstream effects of oxidative damage. The redox status of neutrophils sourced from synovial fluid (SF) was measured by flow cytometry in terms of total ROS and hydroxyl radicals. Among the molecular damage markers, protein carbonylation and lipid peroxidation were detected by spectrophotometry and S-nitrosothiols by fluorimetry. Neutrophils constituted the major cellular component of the SF of patients with RA and their levels of ROS and hydroxyl radicals correlated strongly with protein carbonylation and lipid peroxidation. However, all the oxidative damage markers correlated positively with DAS28. Taken together, in patients with RA, the strong correlation between levels of ROS and DAS28 with markers of oxidative damage suggests that measurement of oxidative stress could serve as a biomarker for monitoring disease severity in RA.