PROPAGATION IN TISSUE CULTURES OF CYTOPATHOGENIC AGENTS FROM PATIENTS WITH MEASLES
PROPAGATION IN TISSUE CULTURES OF CYTOPATHOGENIC AGENTS FROM PATIENTS WITH MEASLES
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DOI：
10.3181/00379727-86-21073
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发表时间：
1954-01-01
影响因子：
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通讯作者：
HOLLOWAY, A
中科院分区：
文献类型：
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作者：
ENDERS, JF;PEEBLES, TC;HOLLOWAY, A
从5例典型麻疹患者的血液或咽洗液中分离出8种具有病毒特性的病原体。药物在体外的增殖伴随着细胞的特征性变化。首先是合胞体巨细胞的形成，其中染色质位于边缘位置，中央被未知性质的嗜酸性物质取代。至少1种药物的致细胞病变作用可被来自其他麻疹患者的恢复期麻疹血清抑制。抗原出现在麻疹病原体的体外培养过程中，在与恢复期麻疹血清的补体结合试验中发生特异性反应。
Eight agents exhibiting the properties of viruses were isolated in cultures of human or simian renal cells from blood or throat washings of 5 cases of typical measles. Multiplication of the agents in vitro is accompanied by characteristic changes in the cells. Primarily these consist in formation of syncytial giant cells wherein the chromatin assumes a marginal position and is replaced centrally by an acidophilic substance of unknown nature. The cytopatho-genic effect of at least 1 of the agents is inhibited by convalescent phase measles sera from other patients with measles. Antigen appears during cultivation in vitro of the measles agents that reacts specifically in complement fixation tests with convalescent phase measles sera.