Flexible and disposable paper- and plastic-based gel micropads for nematode handling, imaging, and chemical testing
Flexible and disposable paper- and plastic-based gel micropads for nematode handling, imaging, and chemical testing
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DOI：
10.1063/1.5005829
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发表时间：
2017-12-01
影响因子：
6
通讯作者：
Pandey, Santosh
中科院分区：
文献类型：
--
作者：
Njus, Zach;Kong, Taejoon;Pandey, Santosh
今天，即时诊断领域与纸微流体技术同义，正在为各种化学测试开发廉价、一次性和现场检测工具包。在这项工作中，我们提出了一种新的应用微流控纸为基础的分析设备（亩PAD），研究一个小的模式线虫，秀丽隐杆线虫的行为。我们描述了在纸和塑料基底上制造μ PAD的方案，其中在琼脂糖和Pluronic凝胶中创建膜。方法被证明用于装载、可视化和转移单个和多个线虫。使用驱虫药左旋咪唑，我们表明，化学测试C。由于开放的器件结构，很容易实现elegans。编写了一个自定义程序来自动识别μ PAD上的单个蠕虫并实时提取运动参数。μ PAD和线虫追踪程序的组合为非微流体线虫实验室提供了相对低成本、易于制造的成像和筛选测定（与标准琼脂糖板或聚合物微流体装置相比）。(C)2017年作者。
Today, the area of point-of-care diagnostics is synonymous with paper microfluidics where cheap, disposable, and on-the-spot detection toolkits are being developed for a variety of chemical tests. In this work, we present a novel application of microfluidic paper-based analytical devices (mu PADs) to study the behavior of a small model nematode, Caenorhabditis elegans. We describe schemes of mu PAD fabrication on paper and plastic substrates where membranes are created in agarose and Pluronic gel. Methods are demonstrated for loading, visualizing, and transferring single and multiple nematodes. Using an anthelmintic drug, levamisole, we show that chemical testing on C. elegans is easily performed because of the open device structure. A custom program is written to automatically recognize individual worms on the a mu PADs and extract locomotion parameters in real-time. The combination of mu PADs and the nematode tracking program provides a relatively low-cost, simple-to-fabricate imaging and screening assay (compared to standard agarose plates or polymeric microfluidic devices) for non-microfluidic, nematode laboratories. (C) 2017 Author(s).