Conjugate addition reactions combined with free-radical cross-linking for the design of materials for tissue engineering
Conjugate addition reactions combined with free-radical cross-linking for the design of materials for tissue engineering
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DOI：
10.1021/bm0056299
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发表时间：
2001-06-01
影响因子：
6.2
通讯作者：
Hubbell, JA
中科院分区：
文献类型：
--
作者：
Elbert, DL;Hubbell, JA
合成了聚乙二丙烯酰胺，并将其用于组织工程材料。丙烯酰胺基团很容易与硫醇基团反应，含有单一硫醇基团的多肽在室温下在水溶液中偶联约2小时，如果只有一部分丙烯酰胺基团与多肽反应，则仍有足够数量的聚乙二丙烯酰胺基团通过自由基光引发聚合。光聚合步骤可以在与细胞接触的情况下进行，为组织工程提供了一种生产生物活性支架的方法。光聚合条件和前驱体组成对材料的刚性有很大影响，从而影响细胞的扩展。测量了细胞在生物活性材料上的铺展动力学和铺展程度，并与组织培养聚苯乙烯上的细胞铺展进行了比较。尽管聚乙二醇材料抵抗蛋白质吸附，但实验表明细胞可以分泌能够附着在凝胶上的细胞外基质。
PEG-diacrylamide was synthesized and utilized to make materials for tissue engineering. Acrylamide groups readily react with thiol groups, and peptides containing a single thiol group were coupled to the PEG-diacrylamide in aqueous solution at room temperature in about 2 h. If only a portion of the acrylamide groups were targeted for reaction with peptide, sufficient amounts of PEG-diacrylamide remained to be polymerized by free-radical mechanisms via photoinitiation. The photopolymerization step can be performed in contact with cells, providing a means to produce bioactive scaffolds for tissue engineering. The photopolymerization conditions and precursor composition greatly affect the stiffness of the materials, which subsequently affected cell spreading. The kinetics and extent of cell spreading on the bioactive materials were measured and compared to cell spreading on tissue culture polystyrene. Although the PEG materials resist protein adsorption, the experiments suggest that the cells can secrete extracellular matrix that can adhere to the gels.