Reactive oxygen species can modulate circadian phase and period in Neurospora crassa
Reactive oxygen species can modulate circadian phase and period in Neurospora crassa
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DOI：
10.1016/j.freeradbiomed.2012.12.016
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发表时间：
2013-05-01
影响因子：
7.4
通讯作者：
Kaldi, Krisztina
中科院分区：
文献类型：
--
作者：
Gyoengyoesi, Norbert;Nagy, Dora;Kaldi, Krisztina
活性氧（ROS）可以作为将代谢与其他细胞功能耦合的信号。除了作为正常新陈代谢的副产品外，它们在环境压力情况下也会以较高的水平产生。我们分析了活性氧如何影响模式生物粗糙脉孢菌的生物钟。在光/暗循环中，活性氧水平的增加提前了分生孢子节律和时钟基因频率表达的阶段。我们的结果表明超氧阴离子在相控制中起主导作用。超氧化物产生的升高导致蛋白磷酸酶 2A 的激活，蛋白磷酸酶 2A 是生物钟正向元素的调节因子。我们的数据表明，即使在无压力条件下，活性氧也会影响昼夜节律。其基础水平的降低会导致光/暗循环中的相位延迟以及恒定条件下更长的周期。我们表明，在夹带条件下，相取决于温度和活性氧物种有助于这种效应。我们的结果表明，超氧阴离子是控制昼夜节律振荡器的重要因素，并且最有可能通过激活蛋白磷酸酶 2A 来重置时钟，从而调节白领复合体的活性。 (C) 2012 Elsevier Inc. 保留所有权利。
Reactive oxygen species (ROS) may serve as signals coupling metabolism to other cell functions. In addition to being by-products of normal metabolism, they are generated at elevated levels under environmental stress situations. We analyzed how reactive oxygen species affect the circadian clock in the model organism Neurospora crassa. In light/dark cycles, an increase in the levels of reactive oxygen species advanced the phase of both the conidiation rhythm and the expression of the clock gene frequency. Our results indicate a dominant role of the superoxide anion in the control of the phase. Elevation of superoxide production resulted in the activation of protein phosphatase 2A, a regulator of the positive element of the circadian clock. Our data indicate that even under nonstress conditions, reactive oxygen species affect circadian timekeeping. Reduction of their basal levels results in a delay of the phase in light/dark cycles and a longer period under constant conditions. We show that under entrained conditions the phase depends on the temperature and reactive oxygen species contribute to this effect. Our results suggest that the superoxide anion is an important factor controlling the circadian oscillator and is able to reset the clock most probably by activating protein phosphatase 2A, thereby modulating the activity of the White Collar complex. (C) 2012 Elsevier Inc. All rights reserved.