CHANGES IN HEMOCYTES OF Plutella xylostella AFTER PARASITISM BY Diadegma semiclausum
CHANGES IN HEMOCYTES OF Plutella xylostella AFTER PARASITISM BY Diadegma semiclausum
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小菜蛾寄生后小菜蛾血细胞的变化
DOI：
10.1002/arch.20284
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发表时间：
2009-03-01
影响因子：
2.2
通讯作者：
Chen, Xue-xin
中科院分区：
文献类型：
--
作者：
Huang, Fang;Shi, Min;Chen, Xue-xin
本文研究了小菜蛾血细胞在被内寄生蜂寄生后的变化。王冠半闭（Hyplutera：Ichelonidae）.观察到不同时期的原血细胞坏死，而寄生幼虫的成熟血细胞中没有细胞死亡，这晚于每微升总血细胞计数（THC）的下降。寄生后12 h，寄主血淋巴中THC含量急剧下降，随后维持在较低水平。当被寄生幼虫的血细胞在体外培养时，包裹能力被抑制，同时铺展能力也被抑制;但这种作用是短暂的，同时血细胞中氧化酚原的激活也被抑制。与以前的研究结果不同，在我们的研究中，由于原血细胞而不是成熟血细胞的坏死而导致的1，77，血细胞的减少不是导致包囊受损的原因。我们的研究表明，D.半闭叶对造血调节和血细胞免疫反应有一定的影响。小菜蛾幼虫。(C)2008 Wiley Periodicals，Inc.
We examined, the changes of hemocytes in the diamondback moth, Plutella xylostella, (Lepidoptera: Plutellidae), due to parasitism by the endoparasitoid. Diadegma. semiclausum. (Hymenoptera: Ichneumonidae). Necrosis of prohemocytes in different stages was observed while cell death was absent in the mature hemocytes in the parasitized larvae, which was,)belated to the declined total hemocyte count per microliter (THC). THC in the host, hemolymph declined sharply by 12h post-parasitization and then remained at a low level. When hemocytes of the parasitized larvae were cultured in vitro, encapsulation ability was suppressed coincidently with the inhibited spreading ability; however, such effects were transient Simultaneously, activation of the prophenoloxidae from the hemocytes was inhibited. Unlike the results of previous studies, the decrease 1,77, hemocytes, which was due to the necrosis of the prohemocytes instead of the mature hemocytes in our study, was not responsible for the impaired encapsulation. Our studies suggest that parasitism by D. semiclausum have some effects on hematopoietic regulation and on hemocyte immune reaction. of P. xylostella, larvae. (C) 2008 Wiley Periodicals, Inc.