The decrease of NMDAR subunit expression and NMDAR EPSC in hippocampus by neonatal exposure to desflurane in mice
The decrease of NMDAR subunit expression and NMDAR EPSC in hippocampus by neonatal exposure to desflurane in mice
复制标题
新生小鼠暴露于地氟烷导致海马 NMDAR 亚基表达和 NMDAR EPSC 降低。
DOI：
10.1016/j.bbr.2016.09.035
复制
发表时间：
2017-01-15
影响因子：
2.7
通讯作者：
Wang, Guolin
中科院分区：
文献类型：
--
作者：
Xu, Xinyu;Zheng, Chen;Wang, Guolin
地氟醚是第三代吸入麻醉剂之一，可用于产科和儿科医学。然而，地氟醚对新生儿大脑的影响在很大程度上是未知的。本研究将6日龄C57BL/6J小鼠分别暴露于1 MAC或1.5 MAC地氟醚中2小时。28日龄时，分别进行开场、自发交替y迷宫和恐惧条件反射测试，评估小鼠的一般活动、工作记忆和长期记忆。western blot检测海马组织中NMDAR亚基NR1、NR2A和NR2 B的表达水平。采用全细胞膜片钳法记录小鼠海马片nmdar介导的兴奋性突触后电流（EPSC）。暴露于1.5 MAC地氟醚的小鼠工作记忆和恐惧条件反射记忆显著受损。NMDAR亚基NR1、NR2B及NMDAR介导的海马EPSC蛋白表达均显著降低。然而，在1.0 MAC地氟醚暴露的小鼠和对照小鼠之间没有发现显著差异。综上所述，在动物模型中，暴露于1.5 MAC地氟醚的6日龄小鼠在出生后28天出现了工作记忆和情境恐惧记忆的显著损伤，海马NMDAR亚基表达和NMDAR EPSC的减少可能参与了这一过程。(C) 2016年Elsevier B.V.出版
Desflurane is one of the third generation inhaled anesthetics and can be used in obstetric and pediatric medicine. However, effects of exposure to desflurane on neonatal brain are largely unknown. In this work, 6-day-old C57BL/6J mice were exposed to 1 MAC or 1.5 MAC desflurane for 2 h. When the mice were 28 day-old, the open-field, spontaneous alternation Y-maze and fear conditioning tests were performed to evaluate general activity, working memory and long term memory, respectively. Levels of NMDAR subunits NR1, NR2A, and NR2 B expression in hippocampus were evaluated by western blot. NMDAR-mediated excitatory postsynaptic current (EPSC) in mouse hippocampal slice was recorded by whole-cell patch clamp record. Mice exposed to 1.5 MAC desflurane had significantly impaired working memory and fear conditioning memory. The protein expression of NMDAR subunits (NR1, NR2B) and NMDAR-mediated EPSC in hippocampus were significantly decreased. However no significant difference was detected between mice exposed to 1.0 MAC desflurane and control mice. In conclusion, in an animal model, 6-day-old mice exposed to 1.5 MAC desflurane have significant impairments in working memory and contextual fear memory at postnatal day 28, and the decrease of NMDAR subunits expression and NMDAR EPSC in hippocampus may be involved in this process. (C) 2016 Published by Elsevier B.V.