Evolution of the MAGUK protein gene family in premetazoan lineages.
Evolution of the MAGUK protein gene family in premetazoan lineages.
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DOI：
10.1186/1471-2148-10-93
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发表时间：
2010-04-01
影响因子：
3.4
通讯作者：
Ruiz-Trillo I
中科院分区：
文献类型：
--
作者：
de Mendoza A;Suga H;Ruiz-Trillo I
细胞间的通讯是多细胞生物的一个关键过程。在多细胞动物中，属于膜相关鸟苷酸激酶（MAGUK）家族的支架蛋白参与细胞连接的调节和形成。这些MAGUK蛋白被认为是后生动物所独有的。然而，最近在Capsaspora owczarzaki的EST调查中发现了MAGUK基因，Capsaspora owczarzaki是一种在后生动物分支附近分支的单细胞生物。为了进一步研究MAGUK的进化历史，我们已经进行了更广泛的搜索，这个基因家族使用不同的后角类的基因组序列。我们的调查和系统发育分析表明，MAGUK蛋白不仅存在于后生动物，但也在choanoflagellate Monosiga brevicollis和原生生物Capsaspora owczarzaki。然而，MAGUK不存在于真菌、变形虫或任何其他真核生物中。MAGUKs在Placozoa和真后生动物分类群（刺胞动物+双体动物）的库是非常相似的，除了一类是在Trichoplax失踪，而Porifera有一个更简单的MAGUK库。然而，Vertebrata经历了几次独立的复制，并展示了两个独家MAGUK类。在两个M. brevicollis和C. owczarzaki：DLG、MPP和MAGI。此外，M。短颈龙经历了谱系特异性的多样化。MAGUK蛋白质基因家族的多样化发生，最有可能的是，在后生动物+choanoflagellates和Capsaspora+Ministeria分支之间的分歧。MAGI样，DLG样和MPP样的祖先基因已经存在于后生动物的单细胞祖先中，新的基因成员已经通过后生动物的进化在两个主要时期内，一个在海绵真后生动物分裂之前，另一个在脊椎动物谱系内。此外，领鞭毛虫遭受了独立的MAGUK多样化。这项研究强调了从尽可能广泛的分类学采样中产生足够的基因组数据的重要性，以充分了解主要蛋白质基因家族的进化历史。
Cell-to-cell communication is a key process in multicellular organisms. In multicellular animals, scaffolding proteins belonging to the family of membrane-associated guanylate kinases (MAGUK) are involved in the regulation and formation of cell junctions. These MAGUK proteins were believed to be exclusive to Metazoa. However, a MAGUK gene was recently identified in an EST survey of Capsaspora owczarzaki, an unicellular organism that branches off near the metazoan clade. To further investigate the evolutionary history of MAGUK, we have undertook a broader search for this gene family using available genomic sequences of different opisthokont taxa. Our survey and phylogenetic analyses show that MAGUK proteins are present not only in Metazoa, but also in the choanoflagellate Monosiga brevicollis and in the protist Capsaspora owczarzaki. However, MAGUKs are absent from fungi, amoebozoans or any other eukaryote. The repertoire of MAGUKs in Placozoa and eumetazoan taxa (Cnidaria + Bilateria) is quite similar, except for one class that is missing in Trichoplax, while Porifera have a simpler MAGUK repertoire. However, Vertebrata have undergone several independent duplications and exhibit two exclusive MAGUK classes. Three different MAGUK types are found in both M. brevicollis and C. owczarzaki: DLG, MPP and MAGI. Furthermore, M. brevicollis has suffered a lineage-specific diversification. The diversification of the MAGUK protein gene family occurred, most probably, prior to the divergence between Metazoa+choanoflagellates and the Capsaspora+Ministeria clade. A MAGI-like, a DLG-like, and a MPP-like ancestral genes were already present in the unicellular ancestor of Metazoa, and new gene members have been incorporated through metazoan evolution within two major periods, one before the sponge-eumetazoan split and another within the vertebrate lineage. Moreover, choanoflagellates have suffered an independent MAGUK diversification. This study highlights the importance of generating enough genome data from the broadest possible taxonomic sampling, in order to fully understand the evolutionary history of major protein gene families.
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影响因子：
3.7
作者：
Shalchian-Tabrizi K;Minge MA;Espelund M;Orr R;Ruden T;Jakobsen KS;Cavalier-Smith T
通讯作者：
Cavalier-Smith T
影响因子：
2.2
作者：
Ruiz-Trillo, Inaki;Lane, Christopher E.;Roger, Andrew J.
通讯作者：
Roger, Andrew J.
影响因子：
2.3
作者：
Aoki, C;Miko, I;Bredt, DS
通讯作者：
Bredt, DS
影响因子：
5.8
作者：
Stamatakis, Alexandros
通讯作者：
Stamatakis, Alexandros
影响因子：
10.7
作者：
Le, Si Quang;Gascuel, Olivier
通讯作者：
Gascuel, Olivier