INSULIN RECEPTOR-RELATED RECEPTOR MESSENGER-RIBONUCLEIC-ACID - QUANTITATIVE DISTRIBUTION AND LOCALIZATION TO SUBPOPULATIONS OF EPITHELIAL-CELLS IN STOMACH AND KIDNEYS
INSULIN RECEPTOR-RELATED RECEPTOR MESSENGER-RIBONUCLEIC-ACID - QUANTITATIVE DISTRIBUTION AND LOCALIZATION TO SUBPOPULATIONS OF EPITHELIAL-CELLS IN STOMACH AND KIDNEYS
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DOI：
10.1210/en.136.9.4125
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发表时间：
1995-09-01
期刊：
影响因子：
4.8
通讯作者：
WATT, VM
中科院分区：
文献类型：
--
作者：
MATHI, SK;CHAN, J;WATT, VM
胰岛素受体相关受体（insulin receptor related receptor， IRR）是胰岛素受体家族的一个新成员，最初是通过克隆与胰岛素受体同源的基因组DNA来鉴定的。我们现在使用Northern blot和多种人体组织的聚合酶链反应分析来证明肾脏是IRR基因表达的主要部位。通过Northern blot分析，仅在人类肾脏中检测到IRR转录本（类似于6和2千碱基）。定量竞争性聚合酶链反应分析显示，IRR信使RNA水平分布更为广泛。人类肾脏中的IRR转录本比胸腺、大脑、心脏、胃中的IRR转录本高3- 19倍，比胎盘、骨骼肌和肝脏中的IRR转录本高50倍。原位杂交组织化学分析显示，IRR转录本存在于人类肾脏远端小管内的细胞亚群中，超出远端弯曲小管的最近段。在大鼠胃中，IRR信使RNA定位于胃底粘膜腺体的神经内分泌细胞亚群。IRR转录本在人和大鼠组织中的选择性分布表明，IRR可能介导一种神经内分泌因子的反应，这种神经内分泌因子以高度组织特异性的方式调节细胞功能的选择方面。
A novel member of the insulin receptor family, the insulin receptor-related receptor (IRR), was initially identified by cloning genomic DNA homologous to the insulin receptor. We have now used Northern blot and polymerase chain reaction analyses of a variety of human tissues to demonstrate that the kidney is a major site of IRR gene expression. IRR transcripts (similar to 6 and similar to 2 kilobases) were detected only in human kidney by Northern blot analyses. Quantitative competitive polymerase chain reaction analysis revealed that IRR messenger RNA levels were distributed more widely. IRR transcripts in human kidney were similar to-3- to 19-fold greater than those in thymus, brain, heart, stomach and similar to 50-fold higher than those in placenta, skeletal muscle, and liver. In situ hybridization histochemical analysis revealed that IRR transcripts were present in a subpopulation of cells within distal tubules of human kidney, beyond the most proximal segment of the distal convoluted tubule. In rat stomach, IRR messenger RNA was localized to a subset of neuroendocrine cells in gastric glands of the fundic mucosa. This selective distribution of IRR transcripts in human and rat tissues suggests that IRR may mediate the responses of a neuroendocrine factor involved in regulating select aspects of cell function in a highly tissue-specific manner.