Reversible phosphorylation of a lectin-receptor-like kinase controls xylem immunity.
Reversible phosphorylation of a lectin-receptor-like kinase controls xylem immunity.
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DOI：
10.1016/j.chom.2023.10.017
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发表时间：
2023-11
影响因子：
30.3
通讯作者：
Ran Wang;Chenying Li;Zhiyi Jia;Yaxing Su;Yingfei Ai;Qinghong Li;Xijie Guo;Zeng Tao;Fucheng Lin;Yan Liang
中科院分区：
文献类型：
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作者：
Ran Wang;Chenying Li;Zhiyi Jia;Yaxing Su;Yingfei Ai;Qinghong Li;Xijie Guo;Zeng Tao;Fucheng Lin;Yan Liang
模式识别受体（PRRs）介导对大多数植物病原体的基础抗性。然而，植物的反应可以是细胞类型特异性的，并且木质部免疫的机制在很大程度上仍然未知。我们表明，凝集素受体样激酶LORE有助于木质部的基础电阻在拟南芥感染青枯菌，破坏性的植物病原体，定殖木质部，导致青枯病。在青枯菌感染后，LORE通过残基S761的磷酸化被激活，启动磷酸化继电器，从而激活活性氧的产生和细胞壁木质化。为了防止免疫信号传导的延长激活，LORE募集并磷酸化2C型蛋白磷酸酶LOPP，其使LORE去磷酸化并减弱LORE介导的木质部免疫以维持免疫稳态。LOPP敲除赋予拟南芥和番茄对细菌性枯萎病的抗性而不影响植物生长。因此，我们的研究揭示了木质部免疫的调节机制，涉及受体样激酶的可逆磷酸化。
Pattern-recognition receptors (PRRs) mediate basal resistance to most phytopathogens. However, plant responses can be cell type specific, and the mechanisms governing xylem immunity remain largely unknown. We show that the lectin-receptor-like kinase LORE contributes to xylem basal resistance in Arabidopsis upon infection withRalstonia solanacearum, a destructive plant pathogen that colonizes the xylem to cause bacterial wilt. FollowingR. solanacearuminfection, LORE is activated by phosphorylation at residue S761, initiating a phosphorelay that activates reactive oxygen species production and cell wall lignification. To prevent prolonged activation of immune signaling, LORE recruits and phosphorylates type 2C protein phosphatase LOPP, which dephosphorylates LORE and attenuates LORE-mediated xylem immunity to maintain immune homeostasis. A LOPP knockout confers resistance against bacterial wilt disease in Arabidopsis and tomatoes without impacting plant growth. Thus, our study reveals a regulatory mechanism in xylem immunity involving the reversible phosphorylation of receptor-like kinases.