Interlaboratory reproducibility of relative abundances of ion currents in fast atom bombardment mass spectral data
Interlaboratory reproducibility of relative abundances of ion currents in fast atom bombardment mass spectral data
复制标题
快原子轰击质谱数据中离子流相对丰度的实验室间再现性
DOI：
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发表时间：
1983
期刊：
影响因子：
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通讯作者：
R. Murphy
中科院分区：
文献类型：
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作者：
K. Clay;L. Wåhlin;R. Murphy
通过比较由8个不同实验室分析的同一样品获得的质谱数据，评价了快原子轰击实验中离子丰度的重现性。仪器包括磁扇区和四极分析仪和几种不同类型的原子枪的初级粒子束。一种特殊的快速原子轰击枪被制造出来，它在设计上不包括粒子束中的离子或电子。此外，该原子枪具有0.1eV的束能量扩展，并且可以聚焦。研究中的分子包括双十六烷酰磷脂酰胆碱、缓激肽、氧化型谷胱甘肽、磺基牛磺石胆酸、ATP、NAD+和水苏糖。实验室之间FAB数据的主要差异在于甘油簇离子的绝对信噪比和丰度。尽管存在主要的仪器差异，但分析物相关离子的相对丰度具有很好的重现性。研究中包含的分析物离子的变异系数通常在20-80%范围内，这与这些实验室获得的硬脂酸甲酯电子轰击质谱的变异系数相似。这些数据表明，快速原子轰击质谱法获得的分析物相关离子的相对强度与电子轰击质谱法一样具有重现性。
The reproducibility of ion abundances in the fast atom bombardment experiment was evaluated by comparing mass spectral data obtained with the same sample analysed by eight different laboratories. Instrumentation included magnetic sector and quadrupole analysers and several different types of atom guns for the primary particle beam. A special fast atom bombardment gun was constructed which, by design, did not include ions or electrons in the particle beam. In addition this atom gun had a 0.1 eV beam energy spread and could be focused. The molecules in the study included dihexadecanoyl phosphatidyl choline, bradykinin, oxidized glutathione, sulfotaurolithocholic acid, ATP, NAD+ and stachyose. Major differences in the FAB data between laboratories were in the absolute signal-to-noise and abundance of glycerol-cluster ions. The relative abundances for analyte-related ions were very reproducible in spite of major instrumental differences. The coefficients of variation for analyte-ions included in the study typically ranged from 20–80% which was similar to those found for the electron impact mass spectrum of methyl stearate obtained by these laboratories. These data suggest that the relative intensities of analyte-related ions obtained fast atom bombardment mass spectrometry are as reproducible as electron impact mass spectrometry.