ADIPOSE-TISSUE - CONTRIBUTIONS OF NATURE AND NURTURE TO OBESITY OF AN OBESE MUTANT MOUSE (OB-OB)
ADIPOSE-TISSUE - CONTRIBUTIONS OF NATURE AND NURTURE TO OBESITY OF AN OBESE MUTANT MOUSE (OB-OB)
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DOI：
10.1098/rspb.1977.0014
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发表时间：
1977-01-01
期刊：
影响因子：
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通讯作者：
SOWTER, C
中科院分区：
文献类型：
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作者：
ASHWELL, M;MEADE, CJ;SOWTER, C
1.研究的目的是比较脂肪组织基因型及其环境对突变小鼠C57 B1/6 Job/ob肥胖的影响。2.这些肥胖小鼠已经足够频繁地与C57 B1/6 J背景进行回交，使异体移植成为可能。3.将来自瘦小鼠或肥胖小鼠的附睾或皮下脂肪块移植到受体瘦小鼠和肥胖小鼠的肾包膜下的部位。将移植物留在原位一个月，然后进行组织学检查以测量脂肪细胞直径，由此估计脂肪细胞质量。4.肥胖供体脂肪的脂肪细胞在“瘦环境”中的尺寸减小到“瘦脂肪”的典型尺寸，而移植到“肥胖环境”中的瘦供体脂肪的细胞增加到“肥胖脂肪”的典型尺寸。5.当瘦的和胖的脂肪组织被移植到同一只老鼠的对侧肾脏时，组织获得了其共同宿主的细胞大小特征。6.因此，在确定肥胖inob/obmice中，内部环境是最重要的，脂肪组织的固有特性是次要的。
1. The aim of the investigation was to compare the contributions of the genotype of the adipose tissue and of its environment to the obesity of the mutant mouse C57 B1/6Job/ob. 2. These obese mice had been sufficiently often backcrossed onto the C57 B1/6J background to make alloplastic transplantation possible. 3. Pieces of either epididymal or subcutaneous fat from lean or obese mice were transplanted to a site underneath the kidney capsule of recipient lean and obese mice. The grafts were left in place for a month and then examined histologically to measure fat cell diameters from which fat cell masses were estimated. 4. The fat cells of obese donor fat decrease in size in a ‘lean environment’ to the size typical of ‘lean fat’ while the cells of lean donor fat transplanted into an ‘obese environment’ increase to the size typical of ‘obese fat’. 5. When both lean and obese fat tissue was transplanted onto opposite kidneys of the same mouse, the tissue acquired the cell size characteristic of its common host. 6. The internal environment is thus of paramount importance and inherent properties of adipose tissue are of lesser importance in determining obesity inob/obmice.