A one centimorgan deletion unit on chromosome Xq12 is commonly lost in borderline and invasive epithelial ovarian tumors
A one centimorgan deletion unit on chromosome Xq12 is commonly lost in borderline and invasive epithelial ovarian tumors
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DOI：
10.1038/sj.onc.1201479
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发表时间：
1998-01-15
期刊：
影响因子：
8
通讯作者：
Mok, SC
中科院分区：
文献类型：
--
作者：
Edelson, MI;Lau, CC;Mok, SC
我们应用聚合酶链反应（PCR）串联重复序列扩增技术，研究了卵巢交界性和浸润性上皮性肿瘤中染色体X11.2-q12上等位基因丢失的模式。应用8个横跨Xq11.2-q12的微卫星标记，分析了41例卵巢交界性和65例浸润性肿瘤及其相应的正常组织，在交界性肿瘤中，DXS 1194位点的杂合性丢失（洛）百分比最高（16例提供信息的病例中的4例，25%）和雄激素受体（AR）位点在侵袭性上皮性卵巢肿瘤中（47例信息病例中的18例，38%），在AR基因座洛缺失病例中进行的X染色体活化研究表明，AR基因座的等位基因缺失并不局限于失活等位基因，在交界性和侵袭性卵巢上皮性肿瘤中，一个包括AR基因座并由引物DXS 1161和PGK 1 P1侧翼的1厘摩区域被鉴定为最小的共同丢失区域。
We have used polymerase chain reaction (PCR) amplification of tandem repeats to study the pattern of allelic loss on chromosome X11.2-q12 in borderline and invasive epithelial ovarian tumors, Using eight microsatellite markers spanning Xq11.2-q12, 41 borderline and 65 invasive ovarian tumors, together with their corresponding normal tissues, were analysed, The highest percentage of loss of heterozygosity (LOH) was observed at the DXS1194 locus in borderline tumors (four of 16 informative cases, 25%) and at the androgen receptor (AR) locus in invasive epithelial ovarian tumors (18 of 47 informative cases, 38%), X chromosome activation studies performed in cases with LOH at the AR locus showed that the allelic loss at the AR locus is not confined to the inactive allele, A one centimorgan region including the AR locus and flanked by the primers DXS1161 and PGK1P1 was identified as the smallest common loss region in both borderline and invasive epithelial ovarian tumors.