Variation in the gene encoding a major merozoite surface antigen of the human malaria parasite Plasmodium falciparum.
Variation in the gene encoding a major merozoite surface antigen of the human malaria parasite Plasmodium falciparum.
复制标题
编码人类疟原虫恶性疟原虫主要裂殖子表面抗原的基因变异。
DOI：
10.1093/nar/14.8.3311
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发表时间：
1986
影响因子：
14.9
通讯作者：
Lyon,JA
中科院分区：
文献类型：
--
作者：
Weber,JL;Leininger,WM;Lyon,JA
恶性疟原虫有一种分子量约为195,000的可变表面蛋白，可能与菌株特异性免疫有关。我们已经从CAMP菌株中克隆并测序了编码该抗原的基因的主要部分，并确定了绿豆核酸酶在该基因周围的首选切割位点。这些位点取决于反应条件，但在每pg DNA含有40%甲酰胺和2单位绿豆核酸酶的情况下，完整的基因从染色体上切除。CAMP菌株基因与其他菌株相同基因的比较。通过对现有DNA序列的匹配和DNA杂交，发现了由不同片段分隔的5个同源区域。两个可变区域编码三个氨基酸重复序列，主要是Ser-Gly-Thr和Thr-Glu-Glu。这些发现对抗原功能的影响，以及产生变异的可能机制进行了讨论。
Plasmodium falciparummerozoites have a variable surface protein of about 195,000 molecular weight which may be involved in strain-specific immunity. We have cloned and sequenced a major portion of the gene encoding this antigen from the CAMP strain and have located sites of preferred mung bean nuclease cleavage around the gene. These sites depend on reaction conditions, but at 40% formamide and 2 units of mung bean nuclease per pg DNA, the intact gene was excised from the chromosome. Comparison of the CAMP strain gene with the same gene from other strains ofP. falciparumby matching available DNA sequences and by DNA hybridization revealed five regions of homology separated by divergent segments. Two of the variable regions encoded three amino acid repeats, predominantly Ser-Gly-Thr and Thr-Glu-Glu. Implications of these findings on the function of the antigen, and possible mechanisms for generation of variants are discussed.