Local and cis effects of the H element on expression of odorant receptor genes in mouse
Local and cis effects of the H element on expression of odorant receptor genes in mouse
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DOI：
10.1016/j.cell.2007.06.023
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发表时间：
2007-07-27
期刊：
影响因子：
64.5
通讯作者：
Mombaerts, Peter
中科院分区：
文献类型：
--
作者：
Fuss, Stefan H.;Omura, Masayo;Mombaerts, Peter
在小鼠基因组中有1,200个气味受体（OR）基因，嗅觉感觉神经元被认为只表达一个基因。OR基因选择的机制尚不清楚。一个2.1激酶区域（H元件）相邻的7个OR基因簇已被提出作为一个反式和泛增强OR基因表达。在这里，我们通过基因打靶在小鼠中删除H元件。该缺失废除了邻近H的三个OR基因的家族的表达，并且H在这些基因上顺式操作。H的缺失对四个OR基因的远端组的表达具有分级效应，与基因组距离相称。对位于簇外的OR基因的表达没有明显的影响。我们的研究结果是不一致的假设H作为一个重要的反式作用增强剂的全基因组调控OR基因的表达。
From the similar to 1,200 odorant receptor (OR) genes in the mouse genome, an olfactory sensory neuron is thought to express only one gene. The mechanisms of OR gene choice are not understood. A 2.1 kilobase region (the H element) adjacent to a cluster of seven OR genes has been proposed as a trans- and pan-enhancer for OR gene expression. Here, we deleted the H element by gene targeting in mice. The deletion abolishes expression of a family of three OR genes proximal to H, and H operates in cis on these genes. Deletion of H has a graded effect on expression of a distal group of four OR genes, commensurate with genomic distance. There is no demonstrable effect on expression of OR genes located outside the cluster. Our findings are not consistent with the hypothesis of H as an essential trans-acting enhancer for genome-wide regulation of OR gene expression.