Detection of intra- and interspecific variation of the dry rot fungus Serpula lacrymans by PCR-RFLP and RAPD analysis
Detection of intra- and interspecific variation of the dry rot fungus Serpula lacrymans by PCR-RFLP and RAPD analysis
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PCR-RFLP 和 RAPD 分析检测干腐真菌 Serpula lacrymans 的种内和种间变异
DOI：
10.1007/s10086-003-0588-9
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发表时间：
2004
影响因子：
2.9
通讯作者：
S. Doi
中科院分区：
文献类型：
--
作者：
S. Horisawa;Y. Sakuma;K. Takata;S. Doi
我们研究了一种基于基因型的测定法来区分干腐真菌Serpula lacrymans。从日本北半部的 74 个分离株中提取 DNA，并通过聚合酶链式反应扩增内转录间隔区 (ITS)。使用 TaqI 和 HhaI 两种酶通过限制性片段长度多态性 (RFLP) 检查分离株的基因型。其中 74 个分离株被鉴定为 S.就形态学特征而言，有 5 个分离株被错误鉴定。进行随机扩增多态性DNA（RAPD）分析以检测S.的种内多样性。在日本分离出的lacryman。由于没有观察到地理起源和遗传距离之间的关系，因此S.的种内多样性。 lacrymansis 建议较小。
We investigated a genotype-based assay to discriminate the dry rot fungiSerpula lacrymans. DNAs were extracted from 74 isolates from the northern half of Japan, and internal transcribed spacers (ITS) were amplified by polymerase chain reaction. Genotypes of isolates were checked by restriction fragment length polymorphism (RFLP) using two enzymes,TaqI andHhaI. Among the 74 isolates identified asS. lacrymansin terms of morphologic features, 5 isolates were shown to have been misidentified. Random amplified polymorphic DNA (RAPD) analysis was conducted in order to detect the intraspecific diversity ofS. lacrymansisolated in Japan. Because no relation between geographical origin and genetic distances was observed, the intraspecific diversity ofS. lacrymansis suggested to be small.