MOUSE CHIMAERAS DEVELOPED FROM FUSED EGGS
MOUSE CHIMAERAS DEVELOPED FROM FUSED EGGS
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DOI：
10.1038/190857a0
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发表时间：
1961-01-01
期刊：
影响因子：
64.8
通讯作者：
TARKOWSKI, AK
中科院分区：
文献类型：
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作者：
TARKOWSKI, AK
8-细胞期晚期的小鼠卵子在培养过程中与另一个品系的卵子融合24或36-40小时，然后在囊胚期移植到受体♀的输卵管或子宫中。12-16小时后。在培养过程中，卵之间的边界(透明带已被移除)完全消失，每对卵都发育出一个没有双重来源痕迹的桑椹。24小时后。几乎所有的桑囊胚都变成了异常大的囊胚，每个囊胚都有一个囊腔。尽管怀孕第19天剖腹产取出的幼崽以及自然出生的幼崽在形态上看起来是正常的，但随后的死亡率极高。导致如此高的产后死亡率的因素尚不清楚。在14名可供检查的年轻人中，2名为♀♀，9名为♂♂，3名为中间性。从这一比例可以得出结论，一些明显的♂♂可能是性嵌合体，其中♂组分占主导地位。嵌合体的发生有以下证据：(1)两个卵子完全结合成一个囊胚；(2)存在两性；(3)大多数由有色和无色融合卵子发育而成的胚胎中存在黑色色素。
Mouse eggs in a late phase of the 8-cell stage were each caused to fuse with an egg of another strain during culture for 24 or 36-40 hrs., and were then transplanted at the blastocyst stage to the oviduct or uterus of a recipient ♀. After 12-16 hrs. in culture, the boundary between the eggs (from which the zona pellucida had been removed) had completely disappeared and a single morula showing no trace of dual origin had developed from each pair. After 24 hrs. nearly all the morulae had turned into unusually large blastocysts, each with a single blastocoelic cavity. Although young removed by Caesarean section on the 19th day of pregnancy, as well as those born naturally, appeared to be morphologically normal, the subsequent mortality rate was extremely high. The factors responsible for this high post-natal mortality are obscure. Of the 14 young available for examination, 2 were ♀♀, 9 were ♂♂ and 3 were intersexes. It is concluded from this ratio that some of the apparent ♂♂ may be sex chimaeras in which the ♂ component dominates. The following evidence was obtained for the occurrence of chimaerism: (1) the complete union of 2 eggs into 1 blastocyst; (2) the presence of inter-sexes; and (3) the presence of black pigment in the majority of embryos developed from pigmented plus non-pigmented fused eggs.