Expression of ZO-1 and occludin in normal human placenta and in hydatidiform moles
Expression of ZO-1 and occludin in normal human placenta and in hydatidiform moles
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DOI：
10.1093/molehr/7.3.279
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发表时间：
2001-03-01
影响因子：
4
通讯作者：
Castellucci, M
中科院分区：
文献类型：
--
作者：
Marzioni, D;Banita, M;Castellucci, M
闭锁小带-1(ZO-1)和闭锁蛋白是细胞间接触的关键分子。它们是紧密连接的成分，因此在组织分化和器官发生中起着关键作用。在本报告中，我们用免疫组织化学和Western印迹分析方法研究了ZO-1和occludin在正常人胎盘和葡萄胎中的表达。在正常胎盘中，ZO-1和occludin主要分布在合体的顶端、合体与绒毛细胞滋养细胞之间的细胞-细胞接触处以及绒毛细胞滋养细胞之间，细胞岛和细胞柱的绒毛外细胞滋养细胞在绒毛基质近侧的细胞层中ZO-1和occludin呈阳性表达，而位于绒毛基质远端的细胞滋养细胞ZO-1和occludin均为阴性，而且整个妊娠过程中胎儿血管均呈ZO-1阳性染色，而occludin阳性反应主要发生在足月，其显著结果是ZO-1在部分和完全葡萄胎中的表达发生变化。在11个葡萄胎中，这两个分子完全不表达，而在另外9个葡萄胎中，它们仅在合胞体和绒毛细胞滋养层细胞中表达，这些结果表明ZO-1和occludin参与了正常的胎盘发育，维持了不同组织成分的组织和功能。这两个分子的下调和/或失调可能与部分性和完全性葡萄胎绒毛上皮细胞转化相关的表型变化有关。
Zonula occludens-1 (ZO-1) and occludin are key molecules in cell-cell contacts. They are tight junction constituents and therefore play a pivotal role in tissue differentiation and organogenesis. In the present report-we have investigated the expression of ZO-1 and occludin in normal human placentae and in hydatidiform moles using immunohistochemical and Western blot analyses. In normal placentae, ZO-1 and occludin were mainly localized in the apical part of the syncytium, in cell-cell contacts between syncytium and villous cytotrophoblastic cells as well as between the latter, Extravillous cytotrophoblast of cell islands and cell columns was positive for ZO-1 and occludin in the cell layers proximally located to the villous stroma whereas the cytotrophoblastic cells, distally located from the villous stroma, were totally negative, Furthermore, fetal vessels showed a positive staining pattern for ZO-1 throughout gestation, whereas a positive reaction for occludin was produced mainly at term, A striking result was the altered expression of ZO-1 and occludin in partial and complete moles. In 11 moles, these two molecules were not expressed at all, while in the other nine, their expression was only cytoplasmic in syncytium and villous cytotrophoblastic cells, These findings suggest that ZO-1 and occludin participate in normal placental development, maintaining the organization and functions of different tissue components. The down-regulation and/or dysregulation of these two molecules may be related to phenotypic changes associated with epithelial cell transformation of the chorionic villi in partial and complete moles.