Regulation of lymphoid-specific immunoglobulin mu heavy chain gene enhancer by ETS-domain proteins.
Regulation of lymphoid-specific immunoglobulin mu heavy chain gene enhancer by ETS-domain proteins.
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ETS 结构域蛋白对淋巴特异性免疫球蛋白 mu 重链基因增强子的调节。
DOI：
10.1126/science.8316859
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Sen,R
中科院分区：
文献类型：
--
作者：
Nelsen,B;Tian,G;Erman,B;Gregoire,J;Maki,R;Graves,B;Sen,R
免疫球蛋白μ重链基因（IgH）的增强子在B淋巴细胞分化的前B细胞阶段激活异源基因。淋巴特异性元件μB是前B细胞中增强子功能所必需的。μB结合蛋白由PU.1/Spi-1原癌基因编码。在结合ets-1原癌基因产物的μ增强子中鉴定出另一个序列元件μA。μA基序是μ B依赖性增强子活性所必需的，这表明最小的B细胞特异性增强子由PU. 1和Ets-1结合位点组成。PU.1和Ets-1在非淋巴细胞中的共表达反式激活了含有最小μ增强子的报告质粒。这些结果暗示Ets家族的两个成员参与了IgH基因表达的激活。
The enhancer for the immunoglobulin μ heavy chain gene (IgH) activates a heterologous gene at the pre-B cell stage of B lymphocyte differentiation. A lymphoid-specific element, μB, is necessary for enhancer function in pre-B cells. A μB binding protein is encoded by thePU.1/Spi-1proto-oncogene. Another sequence element, μA, was identified in the μ enhancer that binds the product of the ets-1 proto-oncogene. The μA motif was required for μB-dependent enhancer activity, which suggests that a minimal B cell-specific enhancer is composed of both the PU.1 and Ets-1 binding sites. Co-expression of both PU.1 and Ets-1 in nonlymphoid cells trans-activated reporter plasmids that contained the minimal μ enhancer. These results implicate two members of the Ets family in the activation ofIgHgene expression.